Abstract：
Polycyclic aromatic hydrocarbons (PAHs) are a cluster of highly hazardous organic pollutants that are widespread in ecosystems and threaten human health. Composting has been shown to be an effective strategy for PAHs degredation. Here, we used Comamonas testosteroni as an inoculant in composting and investigated the potential mechanisms of PAHs degradation by co-occurrence network and structural equation modelling analysis. The results showed that more than 60% of PAHs were removed and the bacterial community responded to the negative effects of PAHs by upgrading the network. Inoculation with C. testosteroni altered bacterial community succession, intensified bacterial response to PAHs, improved metabolic activity, and promoted the degradation of PAHs during co-composting. The increased in the positive cohesion index of the community suggested that agents increased the cooperative behaviour between bacteria and led to changes in keystones of the bacterial network. However, the topological values of C. testosteroni in the network were not elevated, which confirmed that C. testosteroni altered communities by affecting other bacterial growth rather than its own colonisation. This study strengthens our comprehension of the potential mechanisms for the degradation of PAHs in inoculated composting.
摘要：
多环芳烃（PAHs）是一类高度危险的有机污染物，广泛存在于生态系统中，威胁着人类的健康。堆肥已被证明是PAHs降解的有效策略。这里,我们在堆肥中使用了睾丸Coamonas作为接种剂，并通过共生网络和结构方程模型分析研究了PAHs降解的潜在机制。结果表明，超过60％的PAHs被去除，细菌群落通过升级网络来响应PAHs的负面影响。睾丸杆菌接种改变了细菌群落演替，加强细菌对PAHs的反应，改善代谢活动，并促进了共堆肥过程中多环芳烃的降解。社区正内聚指数的增加表明，试剂增加了细菌之间的合作行为，并导致细菌网络基石的变化。然而,网络中睾酮C.的拓扑值没有升高，这证实了C.stestroni通过影响其他细菌的生长而不是其自身的定植来改变群落。这项研究加强了我们对接种堆肥中多环芳烃降解的潜在机制的理解。
