Abstract：
Optical biosensors are frontrunners for the rapid and real-time detection of analytes, particularly for low concentrations. Among them, whispering gallery mode (WGM) resonators have recently attracted a growing focus due to their robust optomechanical features and high sensitivity, measuring down to single binding events in small volumes. In this review, we provide a broad overview of WGM sensors along with critical advice and additional \"tips and tricks\" to make them more accessible to both biochemical and optical communities. Their structures, fabrication methods, materials, and surface functionalization chemistries are discussed. We propose this reflection under a pedagogical approach to describe and explain these biochemical sensors with a particular focus on the most recent achievements in the field. In addition to highlighting the advantages of WGM sensors, we also discuss and suggest strategies to overcome their current limitations, leaving room for further development as practical tools in various applications. We aim to provide new insights and combine different knowledge and perspectives to advance the development of the next generation of WGM biosensors. With their unique advantages and compatibility with different sensing modalities, these biosensors have the potential to become major game changers for biomedical and environmental monitoring, among many other relevant target applications.
摘要：
光学生物传感器是快速和实时检测分析物的前沿，特别是低浓度。其中,耳语画廊模式（WGM）谐振器最近吸引了越来越多的焦点，由于其强大的光机械功能和高灵敏度，测量小卷中的单个结合事件。在这次审查中,我们提供了WGM传感器的广泛概述，以及关键建议和额外的“提示和技巧”，使它们更容易被生化和光学社区访问。他们的结构，制造方法，材料,并讨论了表面功能化化学。我们建议在教学方法下进行这种反思，以描述和解释这些生化传感器，并特别关注该领域的最新成就。除了突出WGM传感器的优点外，我们还讨论并提出克服其当前局限性的策略，作为各种应用的实用工具，为进一步发展留下了空间。我们的目标是提供新的见解，并结合不同的知识和观点，以推进下一代WGM生物传感器的发展。凭借其独特的优势和与不同传感方式的兼容性，这些生物传感器有可能成为生物医学和环境监测的主要游戏规则改变者，在许多其他相关目标应用中。
