PDF(Pubmed)
Abstract：
Pennycress (Thlaspi arvense L.), a member of the Brassicaceae family, produces seed oil high in erucic acid, suitable for biodiesel and aviation fuel. Although pennycress, a winter annual, could be grown as a dedicated bioenergy crop, an increase in its seed oil content is required to improve its economic competitiveness. The success of crop improvement relies upon finding the right combination of biomarkers and targets, and the best genetic engineering and/or breeding strategies. In this work, we combined biomass composition with metabolomic and transcriptomic studies of developing embryos from 22 pennycress natural variants to identify targets for oil improvement. The selected accession collection presented diverse levels of fatty acids at maturity ranging from 29% to 41%. Pearson correlation analyses, weighted gene co-expression network analysis and biomarker identifications were used as complementary approaches to detect associations between metabolite level or gene expression and oil content at maturity. The results indicated that improving seed oil content can lead to a concomitant increase in the proportion of erucic acid without affecting the weight of embryos. Processes, such as carbon partitioning towards the chloroplast, lipid metabolism, photosynthesis, and a tight control of nitrogen availability, were found to be key for oil improvement in pennycress. Besides identifying specific targets, our results also provide guidance regarding the best timing for their modification, early or middle maturation. Thus, this work lays out promising strategies, specific for pennycress, to accelerate the successful development of lines with increased seed oil content for biofuel applications.
摘要：
Pennycress(ThlaspiarvenseL.),十字花科的一员,产生富含芥酸的种子油，适用于生物柴油和航空燃料。虽然pennycress,一年一度的冬天，可以作为专用的生物能源作物种植，为了提高其经济竞争力，需要增加其种子油含量。作物改良的成功依赖于找到生物标志物和靶标的正确组合，以及最好的基因工程和/或育种策略。在这项工作中,我们将生物量组成与来自22个pennycress天然变体的发育胚胎的代谢组学和转录组学研究相结合，以确定改善油脂的目标。选定的登录集在成熟时呈现不同水平的脂肪酸，范围从29％到41％。皮尔逊相关分析，加权基因共表达网络分析和生物标志物鉴定被用作检测成熟时代谢物水平或基因表达与油含量之间关联的补充方法.结果表明，提高种子油含量可以导致芥酸比例的同时增加，而不会影响胚胎的重量。Processes,比如向叶绿体分配碳，脂质代谢，光合作用,严格控制氮的供应，被发现是改良pennycress油的关键。除了确定具体的目标，我们的结果还提供了有关其修改的最佳时机的指导，早期或中期成熟。因此，这项工作提出了有希望的策略，专门针对pennycress，加速成功开发用于生物燃料应用的种子油含量增加的生产线。
