Abstract：
Circular replication-associated protein (Rep)-encoding single stranded (CRESS) DNA viruses include Circoviruses which have been found in several animal species and in human specimens. Circoviruses are associated with severe disease in pigs and birds and with respiratory and gastrointestinal disorders and systemic disease in dogs. In cats there are only a few anecdotical studies reporting CRESS DNA viruses. In this study, a total of 530 samples (361 sera, 131 stools, and 38 respiratory swabs) from cats, were screened for the presence of CRESS DNA viruses. Overall, 48 (9.0%) of 530 samples tested positive using a pan-Rep PCR. A total of 30 Rep sequences were obtained. Ten sequences of fecal origin were tightly related to each other (82.4-100% nt identity) and more distantly related to mongoose circoviruses (68.3 to 77.2% nt identity). At genome level these circoviruses displayed the highest nt identity (74.3-78.7%) to mongoose circoviruses thus representing a novel circovirus species. Circoviruses from different animal hosts (n = 12) and from humans (n = 8) were also identified. However, six Rep sequences were obtained from serum samples, including canine circoviruses, a human cyclovirus and human and fish-associated CRESS DNA viruses. The presence of these viruses in the sera would imply, to various extent, virus replication in the animal host, able to sustain viremia. Overall, these findings indicate a wide genetic diversity of CRESS DNA viruses in cats and warrant further investigations.
摘要：
编码环状复制相关蛋白（Rep）的单链（CRESS）DNA病毒包括已在几种动物物种和人类标本中发现的圆环病毒。圆环病毒与猪和鸟的严重疾病以及狗的呼吸道和胃肠道疾病和全身性疾病有关。在猫中，只有少数研究报告了CRESSDNA病毒。在这项研究中,共530份样本(361份血清，131个凳子,和38只呼吸拭子）来自猫，筛选CRESSDNA病毒的存在。总的来说,530个样品中的48个(9.0%)使用pan-RepPCR检测为阳性。总共获得30个Rep序列。粪便来源的十个序列彼此紧密相关（82.4-100％nt同一性），并且与猫鹅圆环病毒更远近相关（68.3至77.2％nt同一性）。在基因组水平上，这些圆环病毒与猫鹅圆环病毒具有最高的nt同一性（74.3-78.7％），因此代表了一种新型圆环病毒物种。还鉴定了来自不同动物宿主(n=12)和来自人(n=8)的圆环病毒。然而,从血清样本中获得六个Rep序列，包括犬圆环病毒,人类环状病毒以及与人类和鱼类相关的CRESSDNA病毒。血清中这些病毒的存在意味着,在不同程度上，病毒在动物宿主中复制，能够维持病毒血症。总的来说,这些发现表明猫的CRESSDNA病毒具有广泛的遗传多样性，值得进一步研究。
