Abstract：
Biosynthesis involving multi-enzymatic reactions is usually an efficient and economic method to produce plentiful important molecules. To increase the product yield in biosynthesis, the involved enzymes can be immobilized to carriers for enhancing enzyme stability, increasing synthesis efficiency and improving enzyme recyclability. Hydrogels with three-dimensional porous structures and versatile functional groups are promising carriers for enzyme immobilization. Herein, we review the recent advances of the hydrogel-based multi-enzymatic system for biosynthesis. First, we introduce the strategies of enzyme immobilization in hydrogel, including the pros and cons of the strategies. Then we overview the recent applications of the multi-enzymatic system for biosynthesis, including cell-free protein synthesis (CFPS) and non-protein synthesis, especially high value-added molecules. In the last section, we discuss the future perspective of the hydrogel-based multi-enzymatic system for biosynthesis.
摘要：
涉及多酶反应的生物合成通常是产生大量重要分子的有效且经济的方法。为了提高生物合成中的产品产量，所涉及的酶可以固定在载体上，以增强酶的稳定性，提高合成效率，提高酶的可回收性。具有三维多孔结构和多功能官能团的水凝胶是有前途的酶固定化载体。在这里，我们综述了基于水凝胶的多酶生物合成系统的最新进展。首先,我们介绍了在水凝胶中固定化酶的策略，包括策略的利弊。然后我们概述了多酶系统在生物合成中的最新应用，包括无细胞蛋白质合成（CFPS）和非蛋白质合成，特别是高附加值的分子。在最后一节,我们讨论了基于水凝胶的多酶系统用于生物合成的未来前景。
