Abstract：
BACKGROUND: Polymethylmethacrylate (PMMA) is the most used material for the manufacturing of eye prostheses.
OBJECTIVE: To investigate the cytotoxicity of different cleaning agents for ocular prostheses on human conjunctival cells.
METHODS: Six groups of specimens were created (saline, soap, 4% chlorhexidine, hydrogen peroxide, 1% triclosan, and citronella oil). Three specimens were made for each disinfectant at each disinfection period (1, 7, 15, 30, 60, and 90 days), totaling 108 specimens. Thus, the specimens were disinfected, with different disinfectants, for different periods of time. After each disinfection process, the specimens were washed with sterile distilled water. A human conjunctival cell line was grown on the acrylic resin specimens and then cytotoxicity tests (MTT and Neutral Red (NR)) were performed. A negative control (untreated cell cultures) and positive control (Tween 20) were created. Two-way analysis of variance (ANOVA) and Bonferroni test were performed (p < 0.05).
RESULTS: For the MTT and NR tests, when there was a significant difference between the disinfectant and negative control, the disinfectant generated a significant reduction in cell proliferation most of the time.
CONCLUSIONS: All reductions in cell proliferation caused by the disinfectants were clinically acceptable. All disinfectants tested in this study were found to be non-cytotoxic to human conjunctival cells.
OBJECTIVE: To investigate the cytotoxicity of different cleaning agents for ocular prostheses on human conjunctival cells.
METHODS: Six groups of specimens were created (saline, soap, 4% chlorhexidine, hydrogen peroxide, 1% triclosan, and citronella oil). Three specimens were made for each disinfectant at each disinfection period (1, 7, 15, 30, 60, and 90 days), totaling 108 specimens. Thus, the specimens were disinfected, with different disinfectants, for different periods of time. After each disinfection process, the specimens were washed with sterile distilled water. A human conjunctival cell line was grown on the acrylic resin specimens and then cytotoxicity tests (MTT and Neutral Red (NR)) were performed. A negative control (untreated cell cultures) and positive control (Tween 20) were created. Two-way analysis of variance (ANOVA) and Bonferroni test were performed (p < 0.05).
RESULTS: For the MTT and NR tests, when there was a significant difference between the disinfectant and negative control, the disinfectant generated a significant reduction in cell proliferation most of the time.
CONCLUSIONS: All reductions in cell proliferation caused by the disinfectants were clinically acceptable. All disinfectants tested in this study were found to be non-cytotoxic to human conjunctival cells.
摘要：
背景：聚甲基丙烯酸甲酯（PMMA）是用于制造眼假体的最常用材料。
目的：研究不同人工眼清洗剂对人结膜细胞的细胞毒性。
方法：制作6组标本（生理盐水，肥皂,4%氯己定,过氧化氢,1%三氯生,和香茅油）。在每个消毒期（1、7、15、30、60和90天）为每种消毒剂制作三个标本，共108个标本。因此，标本被消毒,用不同的消毒剂，在不同的时间段。每个消毒过程后，样品用无菌蒸馏水洗涤。在丙烯酸树脂样品上生长人结膜细胞系,然后进行细胞毒性测试(MTT和中性红(NR))。产生阴性对照(未处理的细胞培养物)和阳性对照(Tween20)。进行了双向方差分析(ANOVA)和Bonferroni检验(p<0.05)。
结果：对于MTT和NR测试，当消毒剂和阴性对照之间存在显着差异时，消毒剂在大部分时间内产生细胞增殖的显著减少。
结论：所有由消毒剂引起的细胞增殖减少在临床上是可接受的。发现本研究中测试的所有消毒剂对人结膜细胞无细胞毒性。
目的：研究不同人工眼清洗剂对人结膜细胞的细胞毒性。
方法：制作6组标本（生理盐水，肥皂,4%氯己定,过氧化氢,1%三氯生,和香茅油）。在每个消毒期（1、7、15、30、60和90天）为每种消毒剂制作三个标本，共108个标本。因此，标本被消毒,用不同的消毒剂，在不同的时间段。每个消毒过程后，样品用无菌蒸馏水洗涤。在丙烯酸树脂样品上生长人结膜细胞系,然后进行细胞毒性测试(MTT和中性红(NR))。产生阴性对照(未处理的细胞培养物)和阳性对照(Tween20)。进行了双向方差分析(ANOVA)和Bonferroni检验(p<0.05)。
结果：对于MTT和NR测试，当消毒剂和阴性对照之间存在显着差异时，消毒剂在大部分时间内产生细胞增殖的显著减少。
结论：所有由消毒剂引起的细胞增殖减少在临床上是可接受的。发现本研究中测试的所有消毒剂对人结膜细胞无细胞毒性。
