METHODS: The experiment mainly investigated the antiviral mechanism of SeC by locating the virus in the cell, detecting the generation of ROS, observing the DNA status of the cell, and monitoring the mitochondrial membrane potential.
RESULTS: In the present study, SeC was designed to resist A549 cells infections caused by HAdV-14. SeC could prevent HAdV-14 from causing cell apoptosis-related to DNA damage. SeC significantly inhibited ROS generation and protect the cells from oxidative damage induced by ROS against HAdV-14. SeC induced the increase of antiviral cytokines such as IL-6 and IL-8 by activating the Jak2 signaling pathway, and repaired DNA lesions by suppressing ATR, p53, and PARP signaling pathways.
CONCLUSIONS: SeC might provide an effective selenium species with antiviral properties for the therapies against HAdV-14.
方法:本实验主要通过在细胞中定位病毒来研究SeC的抗病毒机制,检测ROS的产生,观察细胞的DNA状态,监测线粒体膜电位.
结果:在本研究中,SeC被设计为抵抗由HAdV-14引起的A549细胞感染。SeC可以防止HAdV-14引起与DNA损伤相关的细胞凋亡。SeC显著抑制ROS的生成,保护细胞免受ROS诱导的HAdV-14氧化损伤。SeC通过激活Jak2信号通路诱导IL-6和IL-8等抗病毒细胞因子的增加,通过抑制ATR修复DNA损伤,p53和PARP信号通路。
结论:SeC可能为HAdV-14的治疗提供具有抗病毒特性的有效硒物质。