Abstract：
The objective of this study was to determine the effect of salinity on anxiety behavior and liver antioxidant capacity in the guppy (Poecilia reticulata). Guppies were exposed to salinities of 0‰, 5‰, 10‰, 15‰ and 20‰ for acute stress tests, and then we analyzed the activity of antioxidant enzymes at 3, 6, 12, 24, 48, 72 and 96 h. During the experiment, the anxiety behavior of guppy was enhanced at salinities of 10‰, 15‰, and 20‰, as evidenced by a significantly higher latency time for the first passage through the upper part than that of the control group (P < 0.05). CAT activity was highest at 24 h in the treatment with the salinity of 10‰, and SOD and GPX activities were highest at 12 h into the treatment with the salinity of 10‰. The SOD and CAT activities were significantly higher than the control group after 96 h of treatment at different salinities (P < 0.05). The MDA contents of the experimental groups at salinities of 5‰ and 10‰ were not significantly different from the control group after 96 h of treatment (P > 0.05). While the MDA contents of the experimental groups at salinities of 15‰ and 20‰ were still significantly higher than the control group after 96 h of treatment (P < 0.05). The experimental results indicated that elevated salinity could lead to oxidative stress in the guppy, altering their anxiety behavior as well as the activity of antioxidant enzymes. In conclusion, drastic changes in salinity during culture should be avoided.
摘要：
这项研究的目的是确定盐度对孔雀鱼（Poeciliareticulata）的焦虑行为和肝脏抗氧化能力的影响。马虎暴露于0‰的盐度，5‰,10‰,急性压力测试为15‰和20‰，然后我们在3、6、12、24、48、72和96h分析了抗氧化酶的活性。在盐度为10‰时，孔雀鱼的焦虑行为增强，15‰，和20‰，第一次通过上部的潜伏期明显高于对照组（P<0.05）。处理24h时CAT活性最高，盐度为10‰，在盐度为10‰的处理中，SOD和GPX活性在12h时最高。不同盐度处理96h后,SOD和CAT活性均显著高于对照组(P<0.05)。盐分为5‰和10‰时，实验组治疗96h后MDA含量与对照组无明显差异（P>0.05）。处理96h后,各实验组在盐度为15‰和20‰时的MDA含量仍显著高于对照组(P<0.05)。实验结果表明，盐度升高会导致孔雀鱼的氧化应激，改变他们的焦虑行为以及抗氧化酶的活性。总之,应避免培养过程中盐度的急剧变化。
