本研究旨在评估丁香酚和1,8-桉树脑对雌性孔雀鱼(Poeciliareticulata)麻醉的协同作用。实验I评估了0、12.5、25、50和75mg/L丁香酚的浓度以及0、100、200、300和400mg/L的1,8-桉树脑的麻醉诱导和恢复时间。实验二将鱼类分为16个研究组,以不同的浓度将丁香酚和1,8-桉树脑成对组合,根据实验I中化学药品的用量,麻醉结果表明,丁香酚在50和70mg/L的浓度下诱导鱼类麻醉,持续时间为256.5和171.5s,分别。相比之下,1,8-桉树脑没有诱导鱼类麻醉。在组合中,使用12.5mg/L的丁香酚和400mg/L的1,8-桉树脑导致224.5s的鱼麻醉。将丁香酚浓度增加到25mg/L,与300和400毫克/升的1,8-桉树脑联合,在259.0和230.5s的时间诱导鱼麻醉,分别。对于50mg/L的丁香酚与100至400mg/L的1,8-桉树脑联合治疗,鱼在189.5、181.5、166.0和157.5s的时间表现出麻醉。在75mg/L的丁香酚的情况下,鱼在175.5、156.5、140.5和121.5s的时间显示麻醉,分别。测试结果表明,1,8-桉树脑作为单一治疗不能诱导鱼类麻醉。然而,当在含有丁香酚的制剂中补充1,8-桉树脑,鱼表现出明显更快的麻醉诱导(p<0.05)。此外,所有接受麻醉的鱼都能完全恢复,没有任何死亡.然而,麻醉持续时间较短导致恢复时间显著延长.总之,丁香酚和1,8-桉树脑作为麻醉药比单独使用更好,并证明了在孔雀鱼上使用这些麻醉剂的安全性。
This study aimed to evaluate the synergistic effect between eugenol and 1,8-cineole on anesthesia in female
guppy fish (Poecilia reticulata). Experiment I evaluated the concentrations of 0, 12.5, 25, 50, and 75 mg/L of eugenol and 0, 100, 200, 300, and 400 mg/L of 1,8-cineole for times of induction and recovery from anesthesia. Experiment II divided fish into 16 study groups, combining eugenol and 1,8-cineole in pairs at varying concentrations, based on the dosage of the chemicals in experiment I. The results of the anesthesia showed that eugenol induced fish anesthesia at concentrations of 50 and 70 mg/L, with durations of 256.5 and 171.5 s, respectively. In contrast, 1,8-cineole did not induce fish anesthesia. In combination, using eugenol at 12.5 mg/L along with 1,8-cineole at 400 mg/L resulted in fish anesthesia at a time of 224.5 s. Increasing the eugenol concentration to 25 mg/L, combined with 1,8-cineole at 300 and 400 mg/L, induced fish anesthesia at times of 259.0 and 230.5 s, respectively. For treatments with eugenol at 50 mg/L combined with 1,8-cineole at 100 to 400 mg/L, fish exhibited anesthesia at times of 189.5, 181.5, 166.0, and 157.5 s. In the case of eugenol at 75 mg/L, fish showed anesthesia at times of 175.5, 156.5, 140.5, and 121.5 s, respectively. The testing results revealed that 1,8-cineole as a single treatment could not induce fish anesthesia. However, when supplementing 1,8-cineole in formulations containing eugenol, fish exhibited a significantly faster induction of anesthesia (p < 0.05). Furthermore, all fish that underwent anesthesia were able to fully recover without any mortality. However, the shorter anesthesia duration resulted in a significantly prolonged recovery time. In conclusion, eugenol and 1,8-cineole work better together as anesthetics than when used separately, and demonstrated the safety of using these anesthetic agents on
guppy fish.