PDF(Pubmed)
Abstract：
Blue mussels from the genus Mytilus are an abundant component of the benthic community, found in the high latitude habitats. These foundation species are relevant to the aquaculture industry, with over 2 million tonnes produced globally each year. Mussels withstand a wide range of environmental conditions and species from the Mytilus edulis complex readily hybridize in regions where their distributions overlap. Significant effort has been made to investigate the consequences of environmental stress on mussel physiology, reproductive isolation, and local adaptation. Yet our understanding on the genomic mechanisms underlying such processes remains limited. In this study, we developed a multi species medium-density 60 K SNP-array including four species of the Mytilus genus. SNPs included in the platform were called from 138 mussels from 23 globally distributed mussel populations, sequenced using a whole-genome low coverage approach. The array contains polymorphic SNPs which capture the genetic diversity present in mussel populations thriving across a gradient of environmental conditions (~59 K SNPs) and a set of published and validated SNPs informative for species identification and for diagnosis of transmissible cancer (610 SNPs). The array will allow the consistent genotyping of individuals, facilitating the investigation of ecological and evolutionary processes in these taxa. The applications of this array extend to shellfish aquaculture, contributing to the optimization of this industry via genomic selection of blue mussels, parentage assignment, inbreeding assessment and traceability. Further applications such as genome wide association studies (GWAS) for key production traits and those related to environmental resilience are especially relevant to safeguard aquaculture production under climate change.
摘要：
Mytilus属的蓝贻贝是底栖群落的丰富组成部分，在高纬度栖息地发现。这些基础物种与水产养殖业有关，全球每年生产超过200万吨。贻贝可以承受各种环境条件，并且来自Mytilusedulis复合体的物种很容易在其分布重叠的区域杂交。已经做出了巨大的努力来研究环境压力对贻贝生理的影响，生殖隔离，当地适应。然而，我们对这些过程背后的基因组机制的理解仍然有限。在这项研究中,我们开发了一种多物种中密度60KSNP阵列，其中包括四种Mytilus属。平台中包含的SNP来自23个全球分布的贻贝种群的138个贻贝，使用全基因组低覆盖率方法进行测序。该阵列包含多态性SNP，可捕获在环境条件梯度下蓬勃发展的贻贝种群中存在的遗传多样性（〜59KSNP），以及一组已发布和经过验证的SNP，可用于物种鉴定和传染性癌症的诊断（610SNP）。该阵列将允许对个体进行一致的基因分型，促进这些分类群的生态和进化过程的研究。该阵列的应用扩展到贝类水产养殖，通过蓝贻贝的基因组选择为该行业的优化做出贡献，亲子关系分配,近亲繁殖评估和可追溯性。诸如关键生产性状的全基因组关联研究（GWAS）和与环境韧性相关的其他应用尤其与在气候变化下保护水产养殖生产有关。
