Abstract：
The extracellular matrix (ECM) plays an important role in lung health and disease. Collagen is the main component of the lung ECM, widely used for the establishment of in vitro and organotypic models of lung disease, and as scaffold material of general interest for the field of lung bioengineering. Collagen also is the main readout for fibrotic lung disease, where collagen composition and molecular properties are drastically changed and ultimately result in dysfunctional \"scarred\" tissue. Because of the central role of collagen in lung disease, quantification, determination of molecular properties, and three-dimensional visualization of collagen is important for both development and characterization of translational models of lung research. In this chapter, we provide a comprehensive overview on the various methodologies currently available for quantification and characterization of collagen including their detection principles, advantages, and disadvantages.
摘要：
细胞外基质（ECM）在肺部健康和疾病中起着重要作用。胶原蛋白是肺ECM的主要成分，广泛用于建立肺部疾病的体外和器官模型，并作为肺生物工程领域普遍感兴趣的支架材料。胶原蛋白也是纤维化肺病的主要读数，其中胶原蛋白组成和分子特性发生了急剧变化，并最终导致功能失调的“疤痕”组织。由于胶原蛋白在肺部疾病中的重要作用，量化，分子性质的测定，胶原蛋白的三维可视化对于肺研究的转化模型的开发和表征都很重要。在这一章中,我们全面概述了目前可用于胶原蛋白定量和表征的各种方法，包括其检测原理，优势,和缺点。
