背景:沙棘(HippophaerhamnoidesL.)因其营养价值而被中国国家卫生委员会指定为“药食同源”水果。在中国传统的民族医学中,沙棘通常用于治疗不愈合的伤口,如烧伤,疮,还有胃溃疡.目的探讨沙棘种子乙酸乙酯提取物(SBS-EF)对烧伤创面的愈合作用。
目的:本研究的主要目的是确定沙棘种子(SBS)治疗烧伤的最有效药用部位,并探讨其治疗作用的物质基础和机制。
方法:通过MTT法评估SBS-EF不同成分对人皮肤成纤维细胞(HSF)增殖和迁移的影响,划痕试验,transwell分析,和羟脯氨酸分泌分析。SBS-EF在提取物中显示出最大的活性。随后的分析包括网络药理学方法,分子对接研究,超高效液相色谱法UPLC-Orbitrap-Excoveris-120-MS和严重的二度烧伤大鼠模型,以研究SBS-EF的化学成分和潜在的治疗机制。
结果:体外研究证明了SBS-EF在促进HSF生长和迁移方面的功效。UPLC-Orbitrap-Excoris-120-MS分析显示SBS-EF有十个主要成分,类黄酮是主要的化合物,尤其是儿茶素,槲皮素,和山奈酚衍生物。网络药理学和分子对接分析表明,SBS-EF可能通过调节Wnt/β-catenin信号通路发挥其愈合作用。随后的体内实验表明,SBS-EF加速了大鼠烧伤伤口的愈合,皮肤组织中羟脯氨酸表达增加,促进皮肤结构修复,并在21d的时间内增强胶原蛋白的产生和组织。此外,暴露于SBS-EF上调大鼠皮肤组织中的WNT3a和β-catenin,同时下调GSK-3β水平。
结论:SBS-EF的伤口愈合特性归因于其增强HSF生长和迁移的能力,增加皮肤中的羟脯氨酸水平,促进胶原蛋白积累,减少疤痕,并降低皮肤含水量。SBS-EF还可以通过调节Wnt/β-catenin信号通路为烧伤提供治疗益处。其在烧伤大鼠治疗中的有效部位和可能的作用机制证明了这一点。
BACKGROUND: Sea buckthorn (Hippophae rhamnoides L.) has been designated a \"medicine food homology\" fruit by the National Health Commission of China due to its nutritional value. In traditional Chinese ethnomedicine, Hippophae rhamnoides L. is commonly used to treat nonhealing wounds such as burns, sores, and gastric ulcers. The aim of this study was to explore the healing effects of the ethyl acetate extract of sea buckthorn seeds (SBS-EF) on burn wounds.
OBJECTIVE: The primary objectives of this research were to determine the most effective medicinal site of action for treating burns with sea buckthorn seeds (SBS) and to investigate the underlying material basis and mechanisms of their therapeutic effects.
METHODS: The effects of different components of SBS-EF on the proliferation and migration of human skin fibroblasts (HSFs) were evaluated via MTT assays, scratch assays, transwell assays, and
hydroxyproline secretion analysis. SBS-EF displayed the greatest activity amongst the extracts. Subsequent analyses included network pharmacology methodology, molecular docking studies, ultraperformance liquid chromatography UPLC-Orbitrap-Exploris-120-MS and a severe second-degree burn rat model to investigate the chemical constituents and potential therapeutic mechanisms of the SBS-EF.
RESULTS: In vitro studies demonstrated the efficacy of SBS-EF in promoting HSF growth and migration. UPLC-Orbitrap-Exploris-120-MS analysis revealed that SBS-EF had ten major constituents, with flavonoids being the predominant compounds, especially catechin, quercetin, and kaempferol derivatives. Network pharmacology and molecular docking analyses indicated that SBS-EF may exert its healing effects by modulating the Wnt/β-catenin signalling pathway. Subsequent in vivo experiments demonstrated that SBS-EF accelerated burn wound healing in rats, increased
hydroxyproline expression in skin tissue, facilitated skin structure repair, and enhanced collagen production and organisation over a 21 d period. Additionally, exposure to SBS-EF upregulated WNT3a and β-catenin while downregulating GSK-3β levels in rat skin tissue.
CONCLUSIONS: The wound healing properties of SBS-EF were attributed to its ability to enhance HSF growth and migration, increase
hydroxyproline levels in the skin, promote collagen accumulation, reduce scarring, and decrease the skin water content. SBS-EF may also provide therapeutic benefits for burns by modulating the Wnt/β-catenin signalling pathway, as evidenced by its effective site and likely mechanism of action in the treatment of burned rats.