PDF(Pubmed)
Abstract：
The emergence of the three germ layers and the lineage-specific precursor cells orchestrating organogenesis represent fundamental milestones during early embryonic development. We analyzed the transcriptional profiles of over 400,000 cells from 14 human samples collected from post-conceptional weeks (PCW) 3 to 12 to delineate the dynamic molecular and cellular landscape of early gastrulation and nervous system development. We described the diversification of cell types, the spatial patterning of neural tube cells, and the signaling pathways likely involved in transforming epiblast cells into neuroepithelial cells and then into radial glia. We resolved 24 clusters of radial glial cells along the neural tube and outlined differentiation trajectories for the main classes of neurons. Lastly, we identified conserved and distinctive features across species by comparing early embryonic single-cell transcriptomic profiles between humans and mice. This comprehensive atlas sheds light on the molecular mechanisms underlying gastrulation and early human brain development.
摘要：
三个胚层的出现和协调器官发生的谱系特异性前体细胞代表了早期胚胎发育的基本里程碑。我们分析了从受孕后几周（PCW）3至12周收集的14个人类样品中超过400,000个细胞的转录谱，以描绘早期胃泌成和神经系统发育的动态分子和细胞景观。我们描述了细胞类型的多样化，神经管细胞的空间模式，和信号通路可能涉及将表皮细胞转化为神经上皮细胞，然后转化为放射状神经胶质细胞。我们解析了沿着神经管的24个放射状神经胶质细胞簇，并概述了主要神经元类别的分化轨迹。最后,我们通过比较人和小鼠的早期胚胎单细胞转录组特征,确定了不同物种的保守和独特特征.这个全面的图集揭示了原肠胚形成和人类早期大脑发育的分子机制。
