PDF(Pubmed)
Abstract：
Dental polymers are now available as monolithic materials which can be readily used in computer-aided design and computer-aided manufacturing (CAD/CAM) systems. Despite possessing numerous advantages over conventionally produced polymers, the polymers produced by either of these systems fail to exhibit immunity to surface microbial adhesion when introduced into the oral environment, leading to the development of oral diseases. The aim of this study was to analyze the biofilm formation of six microorganisms from the oral cavity and its correlation to the surface characteristics of CAD/CAM dental polymers. A total of ninety specimens were divided into three groups: resin-based composite, polymethyl methacrylate, and polyether ether ketone. The experimental procedure included surface roughness and water contact angle measurements, colony forming unit counting, and scanning electron microscopy analysis of biofilm formed on the surface of the tested materials. The data were analyzed using the Kruskal-Wallis test, with a Dunn\'s post hoc analysis, and one way analysis of variance, with a Tukey\'s post hoc test; the correlation between the measurements was tested using Spearman\'s correlation coefficient, and descriptive statistics were used to present the data. Despite using the same manufacturing procedure, as well as the identical manufacturer\'s finishing and polishing protocols, CAD/CAM dental polymers revealed significant differences in surface roughness and water contact angle, and the increased values of both parameters led to an increase in biofilm formation on the surface of the materials. The CAD/CAM resin-based composite showed the lowest number of adhered microorganisms compared to CAD/CAM polymethyl methacrylate and CAD/CAM polyether ether ketone.
摘要：
牙科聚合物现在可以作为整体材料获得,其可以容易地用于计算机辅助设计和计算机辅助制造(CAD/CAM)系统中。尽管与常规生产的聚合物相比具有许多优点，当引入口腔环境时，由这些系统中的任何一个产生的聚合物都无法表现出对表面微生物粘附的免疫力，导致口腔疾病的发展。这项研究的目的是分析口腔中六种微生物的生物膜形成及其与CAD/CAM牙科聚合物表面特征的相关性。共九十个试样分为三组：树脂基复合材料，聚甲基丙烯酸甲酯,和聚醚醚酮。实验程序包括表面粗糙度和水接触角测量，菌落形成单位计数，和扫描电子显微镜分析在测试材料表面上形成的生物膜。数据采用Kruskal-Wallis检验进行分析,邓恩的事后分析，和单向方差分析，使用Tukey的事后检验；使用Spearman相关系数测试测量之间的相关性，和描述性统计用于提供数据。尽管使用相同的制造程序，以及相同的制造商的精加工和抛光协议，CAD/CAM牙科聚合物显示出表面粗糙度和水接触角的显着差异，并且两个参数的增加的值导致材料表面上生物膜形成的增加。与CAD/CAM聚甲基丙烯酸甲酯和CAD/CAM聚醚醚酮相比，基于CAD/CAM树脂的复合材料显示出最低数量的粘附微生物。
