METHODS: Cell viability was evaluated in human periodontal ligament stem cells (hPDLSCs) using the methyl-thiazol-tetrazolium (MTT) colourimetric assay. Polyethylene tubes containing the tested materials and empty polyethylene tubes (control) were implanted in the subcutaneous tissue of Wistar rats. Cellular (lymphocyte infiltration) and extracellular events (ECM; collagen fibres) were analysed in histological sections. Immunohistochemical (collagen I, osteopontin, bone sialoprotein, bone morphogenetic protein4) analyses were also performed.
RESULTS: At 24, 48 and 72 h, all tested groups showed cell viability similar to control (p > .05). Regarding biocompatibility, all groups showed similar cellular events represented by a slight inflammatory reaction characterized by hyperaemia and a mild lymphocytic inflammatory infiltrate. The analysis of lymphocytes during the time showed a decrease in these cells in the control group and a significant interaction between MTA Angelus and control (p < .001), with MTA Angelus showing a more extensive inflammatory infiltrate. Regarding fibres, an increase in content was observed in all groups during the experimental time (7, 30 and 60 days), however, no difference was detected among the experimental groups (p = .063). After 60 days, the immunoexpression of bone matrix proteins in the MTA Flow group was similar to or higher than that observed in the MTA Angelus and in the control group.
CONCLUSIONS: MTA Flow showed a non-cytotoxic behaviour, biocompatibility and ability to stimulate tissue mineralization.
方法:使用甲基-噻唑-四唑(MTT)比色法在人牙周膜干细胞(hPDLSC)中评估细胞活力。将含有测试材料的聚乙烯管和空聚乙烯管(对照)植入Wistar大鼠的皮下组织中。在组织学切片中分析细胞(淋巴细胞浸润)和细胞外事件(ECM;胶原纤维)。免疫组织化学(胶原蛋白I,骨桥蛋白,骨唾液蛋白,骨形态发生蛋白4)分析也进行了。
结果:在24、48和72小时,所有测试组显示与对照组相似的细胞活力(p>0.05)。关于生物相容性,所有组均表现出相似的细胞事件,表现为轻度炎症反应,表现为充血和轻度淋巴细胞炎症浸润.在此期间的淋巴细胞分析显示,对照组中这些细胞减少,MTAAngelus与对照组之间存在显着的相互作用(p<0.001),MTAAngelus表现出更广泛的炎症浸润。关于纤维,在实验时间(7、30和60天)中,所有组的含量均增加,但实验组之间没有检测到差异(p=0.063)。60天后,MTAFlow组骨基质蛋白的免疫表达与MTAAngelus和对照组相似或更高。
结论:MTAFlow显示出非细胞毒性行为,生物相容性和刺激组织矿化的能力。