PDF(Pubmed)
Abstract：
Legionella is a pathogen that colonizes soils, freshwater, and building water systems. People who are most affected are those with immunodeficiencies, so it is necessary to monitor its presence in hospitals. The purpose of this study was to evaluate the presence of Legionella in water samples collected from hospitals in the Campania region, Southern Italy. A total of 3365 water samples were collected from January 2018 to December 2022 twice a year in hospital wards from taps and showers, tank bottoms, and air-treatment units. Microbiological analysis was conducted in accordance with the UNI EN ISO 11731:2017, and the correlations between the presence of Legionella and water temperature and residual chlorine were investigated. In total, 708 samples (21.0%) tested positive. The most represented species was L. pneumophila 2-14 (70.9%). The serogroups isolated were 1 (27.7%), 6 (24.5%), 8 (23.3%), 3 (18.9%), 5 (3.1%), and 10 (1.1%). Non-pneumophila Legionella spp. represented 1.4% of the total. Regarding temperature, the majority of Legionella positive samples were found in the temperature range of 26.0-40.9 °C. An influence of residual chlorine on the presence of the bacterium was observed, confirming that chlorine disinfection is effective for controlling contamination. The positivity for serogroups other than serogroup 1 suggested the need to continue environmental monitoring of Legionella and to focus on the clinical diagnosis of other serogroups.
摘要：
军团菌是一种在土壤中定居的病原体，淡水,建设供水系统。受影响最大的人是免疫缺陷的人，所以有必要监测它在医院的存在。这项研究的目的是评估从坎帕尼亚地区医院收集的水样中军团菌的存在，意大利南部从2018年1月至2022年12月，每年两次在医院病房从水龙头和淋浴处收集3365个水样，罐底,和空气处理单元。根据UNIENISO11731：2017进行微生物分析，并研究了军团菌的存在与水温和残留氯之间的相关性。总的来说,708个样品(21.0%)检测为阳性。最具代表性的物种是嗜肺乳杆菌2-14(70.9%)。分离的血清群为1（27.7％），6（24.5%），8（23.3%），3（18.9%），5（3.1%），和10（1.1%）。非嗜肺军团菌属。占总数的1.4%。关于温度,大多数军团菌阳性样品是在26.0-40.9°C的温度范围内发现的。观察到残留氯对细菌存在的影响，确认氯消毒对控制污染是有效的。血清群1以外的血清群阳性表明，有必要继续对军团菌进行环境监测，并将重点放在其他血清群的临床诊断上。
