Abstract：
The study of lymphocyte polarization upon antigen encounter typically relies on the random pairing between the cells of interest and a stimulating particle (micro bead) that mimics only some of the properties of the antigen-presenting cells. Here, we show how to build and use a microfluidic chip that allows to multiplex and synchronize the encounter between a lymphocyte and an antigen-presenting object: a functionalized oil-in-water droplet. We also explain how to fabricate and functionalize lipid droplets, an antigen-presenting tool that is, at the same time, deformable, fluid, and spherical.
摘要：
抗原相遇时淋巴细胞极化的研究通常依赖于感兴趣的细胞与仅模拟抗原呈递细胞的一些性质的刺激颗粒(微珠)之间的随机配对。这里,我们展示了如何构建和使用微流控芯片，该芯片允许淋巴细胞和抗原呈递对象之间的多重和同步相遇：功能化的水包油滴。我们还解释了如何制造和功能化脂滴，一种抗原呈递工具，同时,可变形,流体,和球形。
