■脂滴(LD)的破坏与许多代谢疾病相关。螺旋藻,作为一种天然的生物活性膳食补充剂,随着运动训练,可以改善脂质代谢;然而,它们对内脏脂肪组织中LDs调节基因的影响尚不清楚.本研究旨在探讨六周螺旋藻补充和运动训练对LDs调节基因表达的影响。
■将56只雄性Wistar大鼠分为6组:盐水(对照组),控制+螺旋藻(螺旋藻),有氧间歇训练(AIT),AIT+螺旋藻(AIT+螺旋藻),阻力训练和阻力+螺旋藻。补充组每周5天食用500mg/kg螺旋藻。训练组进行AIT(每周5次)和阻力训练(每周3次),共6周。通过实时PCR分析了调节内脏脂肪组织(Zw10,Bscl2,DFCP1,Rab18,Syntaxin18,Acsl3和Plin2)中基因表达的LD。
■螺旋藻和运动训练对Syntaxin18(p=0.69)和DFCP1(p=0)的基因表达没有显着影响。84),ACSL3(p=0.98),或BSCL2(p=0.58)。此外,与对照相比,发现螺旋藻显着减弱Plin2(p=0.01)和Rab18(p=0.01)基因的表达,AIT,和阻力训练小组。然而,抗性训练中Plin2基因表达高于AIT。此外,与对照组相比,螺旋藻降低了内脏脂肪组织中ZW10(p=0.03)基因的表达,AIT,和阻力训练小组。出乎意料的是,补充螺旋藻时,如果没有运动训练,则这些基因的表达会进一步降低。
■螺旋藻补充和运动训练对内脏脂肪组织中LDs调节基因有显著影响。
UNASSIGNED: Disruption of lipid droplets (LDs) is associated with many metabolic diseases. Spirulina, as a natural bioactive dietary supplement, along with exercise training, may improve lipid metabolism; however, their effects on LDs-regulated genes in visceral adipose tissue are still unclear. This study aimed to investigate the effects of six-week Spirulina supplementation along with exercise training on LDs regulating gene expression.
UNASSIGNED: Fifty-six male Wistar rats were divided into six groups: saline (control), control+Spirulina (Spirulina), aerobic interval training (AIT), AIT+ Spirulina (AIT+Spirulina), resistance training and resistance+ Spirulina. The supplement groups consumed 500 mg/kg Spirulina five days per week. The training groups performed AIT (5 times per week) and resistance training (3 times per week) for 6 weeks. LDs regulating genes expression in visceral adipose tissue (Zw10, Bscl2, DFCP1, Rab18, Syntaxin 18, Acsl3, and Plin2) was analyzed by real-time PCR.
UNASSIGNED: Spirulina and exercise training had no significant effects on the gene expression of Syntaxin18 (p=0.69) and DFCP1 (p=0. 84), ACSL3 (p=0.98), or BSCL2 (p=0.58). In addition, Spirulina was found to significantly attenuate the expression of Plin2 (p=0.01) and Rab18 (p=0.01) genes compared to the control, AIT, and resistance training groups. However, Plin2 gene expression was higher in the resistance training than the AIT. Furthermore, Spirulina decreased ZW10 (p=0.03) gene expression in visceral adipose tissue compared to the control, AIT, and resistance training groups. Unexpectedly, Spirulina supplementation decreased the expression of these genes even more when taken without exercise training.
UNASSIGNED: Spirulina supplementation and exercise training have significant effects on LDs-regulated genes in visceral adipose tissue.