体外获能可以更好地了解受精的潜在机制,并开发改进的生殖技术以提高猪的生育率。Tyrodes白蛋白乳酸丙酮酸盐(TALP)和改良的KrebsRingers肉汤(m-KRB)是两种在研究实验中通常用于诱导公猪精子获能的媒介(Cañón-Beltrán等。,热遗传学,198、2023和231;Oberlender等人。,兽医档案,44,2012和201;Sahoo等人。,国际生物大分子杂志,241、2023和124502)。此外,了解猪精子在获能期不同时间的形态和功能变化可能有助于开发新技术,以改善精子质量和增加产仔数。进行这项研究是为了研究Tyrode白蛋白乳酸丙酮酸盐和改良的KrebsRingers肉汤培养基在三个不同孵育时期对HD-K75公猪精子体外获能的影响。四个临床健康的人共有24次射精,10-12月龄HD-K75公猪,选择了在ICAR-全印度协调研究项目(AICRP)中维持的猪。使用便携式假人通过“简单拳头”方法收集精液。精液样本体积为200毫升,选择103×106个精子/ml浓度和70%的初始运动性,并分成两部分悬浮在TALP和m-KRB培养基中,分别,并在37°C下孵育5小时。开创性参数,即。精子活力,在孵育0、3和5小时时,对样品中的质膜完整性和顶体完整性进行了评估。这项研究表明,在活顶体反应(p<.05)和宿主反应(p<.01)精子的培养基之间存在显着差异,而在获能期之间,在过度激活的精子中观察到显着(p<.01)变化,活的顶体反应精子,宿主反应精子,FITC标记的PSA,细胞外蛋白和精子胆固醇。在总磷脂中观察到非显著变化。TALP显示对精子活力的总体效果更好,猪精子的质膜和顶体完整性。从这项研究中,可以得出结论,TALP和m-KRB培养基对诱导HD-K75公猪精子的获能是有益的。TALP介质,然而,对精子活力有更好的影响,猪精子的质膜和顶体完整性。在三个不同的时期中,3小时获能期显著(p<.01)导致精子活力的发生率更高,HD-K75公猪精子的质膜和顶体完整性。
In vitro capacitation allows for a greater understanding of the mechanisms underlying fertilization and the development of improved reproductive techniques for improving fertility rates in porcine. Tyrodes albumin lactate pyruvate (TALP) and modified Krebs Ringers Broth (m-KRB) are two medias that are commonly used in research experiments to induce capacitation in boar spermatozoa (Cañón-Beltrán et al., Theriogenology, 198, 2023 and 231; Oberlender et al., Archivos de Medicina Veterinaria, 44, 2012 and 201; Sahoo et al., International Journal of Biological Macromolecules, 241, 2023 and 124502). Moreover, understanding the morphological and functional changes in boar spermatozoa at different hours of capacitation periods might aid in the development of novel techniques for improving sperm quality and increasing the litter size. This study was carried out to investigate the effect of Tyrode albumin lactate pyruvate and modified Krebs Ringers Broth media on in vitro capacitation of HD-K75 boar spermatozoa at three different periods of incubation. A total of 24 ejaculate from four clinically healthy, 10-12 months aged HD-K75 boars, maintained at ICAR-All India Coordinated Research Project (AICRP) on pig were selected. Semen was collected by \'Simple fist\' method using a portable dummy. The semen samples having 200 mL volume, 103 × 106 spermatozoa/ml concentration and 70% initial motility were selected and split into two parts and suspended in TALP and m-KRB media, respectively, and incubated for 5 h at 37°C. Seminal parameters viz. sperm viability, plasma membrane integrity and acrosomal integrity were estimated in the samples at 0, 3 and 5 h of incubation. This study revealed that there was significant variation between media in live acrosome-reacted (p < .05) and HOST-reacted (p < .01) spermatozoa, while between capacitation periods significant (p < .01) variation was observed in hyperactivated spermatozoa, live acrosome-reacted spermatozoa, HOST-reacted spermatozoa, FITC-labelled PSA, extracellular protein and sperm cholesterol. Non-significant variation was observed in total phospholipid. TALP showed overall better consequence on sperm viability, plasma membrane and acrosomal integrity of boar spermatozoa. From this study, it could be concluded that both TALP and m-KRB media were virtuous to induce capacitation in HD-K75 boar spermatozoa. TALP media, however, had a better effect on sperm viability, plasma membrane and acrosomal integrity of boar spermatozoa. Out of the three different periods, 3 h capacitation period resulted in significantly (p < .01) higher incidence of sperm viability, plasma membrane and acrosomal integrity in HD-K75 boar spermatozoa.