PDF(Pubmed)
Abstract：
Newly synthesized membrane proteins pass through the secretory pathway, starting at the endoplasmic reticulum and packaged into COPII vesicles, to continue to the Golgi apparatus before reaching their membrane of residence. It is known that cargo receptor proteins form part of the COPII complex and play a role in the recruitment of cargo proteins for their subsequent transport through the secretory pathway. The role of cornichon proteins is conserved from yeast to vertebrates, but it is poorly characterized in plants. Here, we studied the role of the two cornichon homologs in the secretory pathway of the moss Physcomitrium patens. Mutant analyses revealed that cornichon genes regulate different growth processes during the moss life cycle by controlling auxin transport, with CNIH2 functioning as a specific cargo receptor for the auxin efflux carrier PINA, with the C terminus of the receptor regulating the interaction, trafficking and membrane localization of PINA.
摘要：
新合成的膜蛋白通过分泌途径，从内质网开始，包装成COPII囊泡，在到达他们的居住膜之前继续到高尔基体。已知货物受体蛋白形成COPII复合物的一部分，并在货物蛋白的募集中发挥作用，以便随后通过分泌途径运输。从酵母到脊椎动物，玉米蛋白的作用是保守的，但它在植物中的特征很差。这里,我们研究了两种玉米同系物在苔藓Physcomitriumpatens分泌途径中的作用。突变体分析表明，在苔藓生命周期中，Cornichon基因通过控制生长素的运输来调节不同的生长过程，CNIH2作为生长素外排载体PINA的特异性货物受体，受体的C端调节相互作用，贩运，和PINA的膜定位。
