苯并[a]芘(BaP)是燃烧过程中产生的多环芳烃化合物,并存在于各种物质中,如食物,烟草烟雾,燃烧排放。BaP被广泛认为是一种高度致癌的物质,可以诱导多种形式的癌症,比如肺癌,皮肤癌,还有胃癌.最近,它被证明对生殖系统产生不利影响。然而,BaP对卵母细胞质量的潜在毒性尚不清楚.在这项研究中,我们通过小鼠口腔灌胃建立了BaP暴露模型,发现BaP暴露导致卵巢重量显着降低,卵巢中GV卵母细胞的数量,和卵母细胞成熟能力。BaP暴露导致核糖体功能障碍,以卵母细胞中RPS3和HPG的表达降低为特征。BaP暴露还引起内质网(ER)的异常分布和诱导ER应激,如GRP78的表达增加所示。此外,高尔基体表现出异常的定位模式,GM130本地化证实了这一点。通过Rab10的异常表达和定位观察到囊泡运输过程的中断。此外,增强的溶酶体和LC3荧光强度表明卵母细胞中蛋白质降解的发生。总之,我们的结果表明,BaP暴露破坏了细胞器的分布和功能,从而影响小鼠卵母细胞的发育能力。
Benzo[a]pyrene (BaP) is a polycyclic aromatic hydrocarbon compound that is generated during combustion processes, and is present in various substances such as foods, tobacco smoke, and burning emissions. BaP is extensively acknowledged as a highly carcinogenic substance to induce multiple forms of cancer, such as lung cancer, skin cancer, and stomach cancer. Recently it is shown to adversely affect the reproductive system. Nevertheless, the potential toxicity of BaP on oocyte quality remains unclear. In this study, we established a BaP exposure model via mouse oral gavage and found that BaP exposure resulted in a notable decrease in the ovarian weight, number of GV oocytes in ovarian, and oocyte maturation competence. BaP exposure caused ribosomal dysfunction, characterized by a decrease in the expression of RPS3 and HPG in oocytes. BaP exposure also caused abnormal distribution of the endoplasmic reticulum (ER) and induced ER stress, as indicated by increased expression of GRP78. Besides, the Golgi apparatus exhibited an abnormal localization pattern, which was confirmed by the GM130 localization. Disruption of vesicle transport processes was observed by the abnormal expression and localization of Rab10. Additionally, an enhanced lysosome and LC3 fluorescence intensity indicated the occurrence of protein degradation in oocytes. In summary, our results suggested that BaP exposure disrupted the distribution and functioning of organelles, consequently affecting the developmental competence of mouse oocytes.