Abstract：
Golgi-derived PI4P-containing vesicles play important roles in mitochondrial division, which is essential for maintaining cellular homeostasis. However, the mechanism of the PI4P-containing vesicle effect on mitochondrial division is unclear. Here, we found that actin appeared to polymerize at the contact site between PI4P-containing vesicles and mitochondria, causing mitochondrial division. Increasing the content of PI4P derived from the Golgi apparatus increased actin polymerization and reduced the length of the mitochondria, suggesting that actin polymerization through PI4P-containing vesicles is involved in PI4P vesicle-related mitochondrial division. Collectively, our results support a model in which PI4P-containing vesicles derived from the Golgi apparatus cooperate with actin filaments to participate in mitochondrial division by contributing to actin polymerization, which regulates mitochondrial dynamics. This study enriches the understanding of the pathways that regulate mitochondrial division and provides new insight into mitochondrial dynamics.
摘要：
高尔基来源的含PI4P的囊泡在线粒体分裂中起重要作用，这对于维持细胞稳态至关重要。然而,含PI4P的囊泡对线粒体分裂的作用机制尚不清楚.这里,我们发现肌动蛋白似乎在含有PI4P的囊泡和线粒体之间的接触位点聚合，导致线粒体分裂.增加来自高尔基体的PI4P的含量增加了肌动蛋白的聚合并减少了线粒体的长度，表明肌动蛋白通过含PI4P的囊泡聚合参与PI4P囊泡相关的线粒体分裂。总的来说，我们的结果支持了一个模型，其中来自高尔基体的含PI4P的囊泡与肌动蛋白丝合作，通过促进肌动蛋白聚合来参与线粒体分裂，调节线粒体动力学。这项研究丰富了对调节线粒体分裂的途径的理解，并为线粒体动力学提供了新的见解。
