Long-term preservation and genetic stability of entomopathogenic fungal species.

昆虫病原真菌物种的长期保存和遗传稳定性。

• 影响指数: 2.622
• 发表时间: 05 2023 2
• 来源期刊: J Microbiol Methods
• DOI: 10.1016/j.mimet.2023.106711
• PMID: 37015312
• 文章类型: Journal Article
• 中科院分区: Q3
• 方向: 生物
• 作者列表: Ayala-Zermeño MA,Berlanga-Padilla AM,Regla-Márquez CF,Lino-López GJ,Muñiz-Paredes F,Montesinos-Matías R,Sánchez-González JA

关键词: Genetic stability; Hypocreales; Long-term conservation; Phenotypic characteristics; Preservation methods

Mesh: Cryopreservation / methods; Freeze Drying / methods; Freezing

Abstract

Entomopathogenic fungi (EPF) preservation aims to maintain valuable characteristics. Growth, conidiation and genetic stability of eight species of EPF were evaluated in six preservation methods for up to 8.2 years. Cryopreservation at -196 °C, freeze-drying, and ultra-freezing at -70 °C resulted as the best methods for long-term storage.

摘要

昆虫病原真菌（EPF）的保存旨在保持有价值的特征。增长,在6种保存方法中，对8种EPF的分生孢子和遗传稳定性进行了长达8.2年的评估。-196°C低温保存,冷冻干燥,在-70°C下超冻是长期储存的最佳方法。