Abstract：
The etiopathogenesis of diverticular disease is unknown.
To compare the fecal and mucosa-associated microbiota between participants with and without diverticulosis and participants who later developed diverticulitis versus those that did not from a population-based study.
The PopCol study, conducted in Stockholm, Sweden, invited a random sample of 3556 adults to participate, of which 745 underwent colonoscopy. Overall, 130 participants (17.5%) had diverticulosis. 16S rRNA gene sequencing was conducted on available sigmoid biopsy samples from 529 and fecal samples from 251 individuals. We identified individuals who subsequently developed acute diverticulitis up to 13 years after sample collection. In a case-control design matching for gender, age (+/-5 years), smoking and antibiotic exposure, we compared taxonomic composition, richness and diversity of the microbiota between participants with or without diverticulosis, and between participants who later developed acute diverticulitis versus those who did not.
No differences in microbiota richness or diversity were observed between participants with or without diverticulosis, nor for those who developed diverticulitis compared with those who did not. No bacterial taxa were significantly different between participants with diverticulosis compared with those without diverticulosis. Individuals who later developed acute diverticulitis (2.8%) had a higher abundance of genus Comamonas than those who did not (p = .027).
In a population-based cohort study the only significant difference was that those who later develop diverticulitis had more abundance of genus Comamonas. The significance of Comamonas is unclear, suggesting a limited role for the gut microbiota in the etiopathogenesis of diverticular disease.
To compare the fecal and mucosa-associated microbiota between participants with and without diverticulosis and participants who later developed diverticulitis versus those that did not from a population-based study.
The PopCol study, conducted in Stockholm, Sweden, invited a random sample of 3556 adults to participate, of which 745 underwent colonoscopy. Overall, 130 participants (17.5%) had diverticulosis. 16S rRNA gene sequencing was conducted on available sigmoid biopsy samples from 529 and fecal samples from 251 individuals. We identified individuals who subsequently developed acute diverticulitis up to 13 years after sample collection. In a case-control design matching for gender, age (+/-5 years), smoking and antibiotic exposure, we compared taxonomic composition, richness and diversity of the microbiota between participants with or without diverticulosis, and between participants who later developed acute diverticulitis versus those who did not.
No differences in microbiota richness or diversity were observed between participants with or without diverticulosis, nor for those who developed diverticulitis compared with those who did not. No bacterial taxa were significantly different between participants with diverticulosis compared with those without diverticulosis. Individuals who later developed acute diverticulitis (2.8%) had a higher abundance of genus Comamonas than those who did not (p = .027).
In a population-based cohort study the only significant difference was that those who later develop diverticulitis had more abundance of genus Comamonas. The significance of Comamonas is unclear, suggesting a limited role for the gut microbiota in the etiopathogenesis of diverticular disease.
摘要：
■憩室病的病因未知。
比较有和没有憩室病的参与者和后来发生憩室炎的参与者与没有发生憩室炎的参与者之间的粪便和粘膜相关微生物群。
■PopCol研究，在斯德哥尔摩进行，瑞典,邀请3556名成年人随机抽样参加，其中745人接受了结肠镜检查。总的来说,130名参与者（17.5％）患有憩室病。对来自529个个体的可用乙状结肠活检样品和来自251个个体的粪便样品进行16SrRNA基因测序。我们确定了在样本收集后13年内随后发展为急性憩室炎的个体。在性别匹配的案例控制设计中，年龄（+/-5岁），吸烟和接触抗生素，我们比较了分类组成，有或没有憩室病的参与者之间微生物群的丰富度和多样性，以及后来发展为急性憩室炎的参与者与未发展为急性憩室炎的参与者之间的关系。
■在有或没有憩室病的参与者之间没有观察到微生物群丰富度或多样性的差异，与未患有憩室炎的人相比，也不适用。与没有憩室病的参与者相比，患有憩室病的参与者之间没有细菌分类单元显着差异。后来发展为急性憩室炎的个体（2.8％）的Comamonas属的丰度高于未发展的个体（p=.027）。
■在一项基于人群的队列研究中，唯一的显著差异是那些后来发展为憩室炎的人有更多的Comamonas属。Comamonas的意义尚不清楚,提示肠道菌群在憩室病的病因中的作用有限。
比较有和没有憩室病的参与者和后来发生憩室炎的参与者与没有发生憩室炎的参与者之间的粪便和粘膜相关微生物群。
■PopCol研究，在斯德哥尔摩进行，瑞典,邀请3556名成年人随机抽样参加，其中745人接受了结肠镜检查。总的来说,130名参与者（17.5％）患有憩室病。对来自529个个体的可用乙状结肠活检样品和来自251个个体的粪便样品进行16SrRNA基因测序。我们确定了在样本收集后13年内随后发展为急性憩室炎的个体。在性别匹配的案例控制设计中，年龄（+/-5岁），吸烟和接触抗生素，我们比较了分类组成，有或没有憩室病的参与者之间微生物群的丰富度和多样性，以及后来发展为急性憩室炎的参与者与未发展为急性憩室炎的参与者之间的关系。
■在有或没有憩室病的参与者之间没有观察到微生物群丰富度或多样性的差异，与未患有憩室炎的人相比，也不适用。与没有憩室病的参与者相比，患有憩室病的参与者之间没有细菌分类单元显着差异。后来发展为急性憩室炎的个体（2.8％）的Comamonas属的丰度高于未发展的个体（p=.027）。
■在一项基于人群的队列研究中，唯一的显著差异是那些后来发展为憩室炎的人有更多的Comamonas属。Comamonas的意义尚不清楚,提示肠道菌群在憩室病的病因中的作用有限。
