Abstract：
Fungal infections are a growing public health concern worldwide and the emergence of antifungal resistance has limited the number of therapeutic options. Therefore, developing novel strategies for identifying and developing new antifungal compounds is an active area of research in the pharmaceutical industry. In this study, we purified and characterized a trypsin protease inhibitor obtained from Yellow Bell Pepper (Capsicum annuum L.) seeds. The inhibitor not only showed potent and specific activity against the pathogenic fungus Candida albicans, but was also found to be non-toxic against human cells. Furthermore, this inhibitor is unique in that it also inhibits α-1,4-glucosidase, positioning it as one of the first plant-derived protease inhibitors with dual biological activity. This exciting discovery opens new avenues for the development of this inhibitor as a promising antifungal agent and highlights the potential of plant-derived protease inhibitors as a rich source for the discovery of novel multifunctional bioactive molecules.
摘要：
真菌感染是全球范围内日益增长的公共卫生问题，抗真菌耐药性的出现限制了治疗选择的数量。因此,开发新的策略来识别和开发新的抗真菌化合物是制药行业研究的一个活跃领域。在这项研究中,我们纯化并表征了从黄铃辣椒（CapsicumannuumL.）种子中获得的胰蛋白酶蛋白酶抑制剂。该抑制剂不仅对病原真菌白色念珠菌显示出有效和特异的活性，但也被发现对人类细胞无毒。此外，这种抑制剂的独特之处在于它还抑制α-1,4-葡萄糖苷酶，将其定位为首批具有双重生物活性的植物源性蛋白酶抑制剂之一。这一令人兴奋的发现为该抑制剂作为有前途的抗真菌剂的开发开辟了新的途径，并突出了植物来源的蛋白酶抑制剂作为发现新型多功能生物活性分子的丰富来源的潜力。
