PDF(Pubmed)
Abstract：
This study aimed to identify the regulatory mechanisms of white, blue, red lights on carotenoid and tocochromanol biosynthesis in mung bean sprouts. Results showed that three lights stimulated the increase of the predominated lutein (3.2-8.1 folds) and violaxanthin (2.1-6.1 folds) in sprouts as compared with dark control, as well as β-carotene (20-36 folds), with the best yield observed under white light. Light signals also promoted α- and γ-tocopherol accumulation (up to 1.8 folds) as compared with dark control. The CRTISO, LUT5 and DXS (1.24-6.34 folds) exhibited high expression levels under light quality conditions, resulting in an overaccumulation of carotenoids. The MPBQ-MT, TC and TMT were decisive genes in tocochromanol biosynthesis, and were expressed up to 4.19 folds as compared with control. Overall, the results could provide novel insights into light-mediated regulation and fortification of carotenoids and tocopherols, as well as guide future agricultural cultivation of mung bean sprouts.
摘要：
本研究旨在明确白,蓝色,红光对绿豆芽中类胡萝卜素和生育酚的生物合成。结果表明，与深色对照相比，三种光刺激了豆芽中主要叶黄素（3.2-8.1倍）和紫黄质（2.1-6.1倍）的增加。以及β-胡萝卜素（20-36倍），在白光下观察到最好的产量。与暗对照相比，光信号还促进了α-和γ-生育酚的积累（高达1.8倍）。CRTISO,LUT5和DXS（1.24-6.34倍）在光质条件下表现出高表达水平，导致类胡萝卜素的过度积累。MPBQ-MT,TC和TMT是生育色满醇生物合成的决定性基因，与对照组相比，其表达量高达4.19倍。总的来说,结果可以提供新的见解光介导的调节和强化类胡萝卜素和生育酚，以及指导未来农业种植绿豆芽。
