Abstract：
BACKGROUND: Chemical control is an important method for tackling crop diseases. Clarifying the antibacterial mechanisms of bactericides is useful for developing new bactericides and for continuous plant disease control. In this study, the antibacterial mechanism of a novel bactericide, dioctyldiethylenetriamine (Xinjunan), which affects adenosine triphosphate (ATP) synthesis, was investigated.
RESULTS: The results of an in vitro inhibition activity assay showed that dioctyldiethylenetriamine inhibited the growth of a variety of plant pathogenic bacteria, especially that of Xanthomonas spp. Scanning electron microscopy demonstrated that dioctyldiethylenetriamine caused cell distortion and rupture. To investigate the molecular mechanism underlying the antibacterial effect of dioctyldiethylenetriamine, transcriptome sequencing (RNA-seq) was performed for Xanthomonas oryzae pv. oryzae (Xoo, PXO99A) treated with dioctyldiethylenetriamine, which has strong antibacterial effects against xanthomonads. The results showed that differentially expressed genes were enriched mainly in the oxidative phosphorylation and tricarboxylic acid (TCA) cycle pathways after treatment. Moreover, the dioctyldiethylenetriamine treatment exhibited reduction in enzyme activities in the TCA cycle, decreased intracellular nicotinamide adenine dinucleotide and ATP contents, and increased accumulation of reactive oxygen species. In addition, dioctyldiethylenetriamine exhibited an inhibitory effect on the growth of other bacterial pathogens by reducing ATP synthesis.
CONCLUSIONS: This is the first report of the mechanism by which dioctyldiethylenetriamine inhibits ATP synthesis by affecting oxidative phosphorylation and TCA cycle pathways in bacteria. © 2023 Society of Chemical Industry.
RESULTS: The results of an in vitro inhibition activity assay showed that dioctyldiethylenetriamine inhibited the growth of a variety of plant pathogenic bacteria, especially that of Xanthomonas spp. Scanning electron microscopy demonstrated that dioctyldiethylenetriamine caused cell distortion and rupture. To investigate the molecular mechanism underlying the antibacterial effect of dioctyldiethylenetriamine, transcriptome sequencing (RNA-seq) was performed for Xanthomonas oryzae pv. oryzae (Xoo, PXO99A) treated with dioctyldiethylenetriamine, which has strong antibacterial effects against xanthomonads. The results showed that differentially expressed genes were enriched mainly in the oxidative phosphorylation and tricarboxylic acid (TCA) cycle pathways after treatment. Moreover, the dioctyldiethylenetriamine treatment exhibited reduction in enzyme activities in the TCA cycle, decreased intracellular nicotinamide adenine dinucleotide and ATP contents, and increased accumulation of reactive oxygen species. In addition, dioctyldiethylenetriamine exhibited an inhibitory effect on the growth of other bacterial pathogens by reducing ATP synthesis.
CONCLUSIONS: This is the first report of the mechanism by which dioctyldiethylenetriamine inhibits ATP synthesis by affecting oxidative phosphorylation and TCA cycle pathways in bacteria. © 2023 Society of Chemical Industry.
摘要：
背景：化学防治是控制作物病害的重要方法。阐明杀菌剂的抗菌机理对于开发新的杀菌剂和连续的植物病害控制是有用的。在这项研究中,一种新型杀菌剂的抗菌机理，二辛基二亚乙基三胺（新村），影响三磷酸腺苷（ATP）合成，被调查。
结果:体外抑制活性测定结果表明,二辛基二亚乙基三胺抑制多种植物病原菌的生长,尤其是黄单胞菌属。扫描电镜显示二辛基二亚乙基三胺引起细胞变形和破裂。探讨二辛基二亚乙基三胺抗菌作用的分子机制,对米黄单胞菌pv进行转录组测序(RNA-seq)。稻米（Xoo，PXO99A）用二辛基二亚乙基三胺处理，对黄蛾有很强的抗菌作用。结果显示,治疗后差异表达基因主要富集在氧化磷酸化和三羧酸（TCA）循环途径中。此外，发现用二辛基二亚乙基三胺处理显示TCA循环中酶活性降低，细胞内烟酰胺腺嘌呤二核苷酸和ATP含量降低，并增加了活性氧的积累。此外,二辛基二亚乙基三胺通过减少ATP合成对其他细菌病原体的生长具有抑制作用。
结论：这是关于二辛基二亚乙基三胺通过影响细菌中的氧化磷酸化和TCA循环途径来抑制ATP合成的机制的首次报道。
结果:体外抑制活性测定结果表明,二辛基二亚乙基三胺抑制多种植物病原菌的生长,尤其是黄单胞菌属。扫描电镜显示二辛基二亚乙基三胺引起细胞变形和破裂。探讨二辛基二亚乙基三胺抗菌作用的分子机制,对米黄单胞菌pv进行转录组测序(RNA-seq)。稻米（Xoo，PXO99A）用二辛基二亚乙基三胺处理，对黄蛾有很强的抗菌作用。结果显示,治疗后差异表达基因主要富集在氧化磷酸化和三羧酸（TCA）循环途径中。此外，发现用二辛基二亚乙基三胺处理显示TCA循环中酶活性降低，细胞内烟酰胺腺嘌呤二核苷酸和ATP含量降低，并增加了活性氧的积累。此外,二辛基二亚乙基三胺通过减少ATP合成对其他细菌病原体的生长具有抑制作用。
结论：这是关于二辛基二亚乙基三胺通过影响细菌中的氧化磷酸化和TCA循环途径来抑制ATP合成的机制的首次报道。
