PDF(Pubmed)
Abstract：
Surrogate viruses theoretically provide an opportunity to study the viral spread in an indoor environment, a highly needed understanding during the pandemic, in a safe manner to humans and the environment. However, the safety of surrogate viruses for humans as an aerosol at high concentrations has not been established. In this study, Phi6 surrogate was aerosolized at high concentration (Particulate matter2.5: ∼1018 μg m-3) in the studied indoor space. Participants were closely followed for any symptoms. We measured the bacterial endotoxin concentration of the virus solution used for aerosolization as well as the concentration in the room air containing the aerosolized viruses. In addition, we measured how the bacterial endotoxin concentration of the sample was affected by different traditional virus purification procedures. Despite the purification, bacterial endotoxin concentration of the Phi6 was high (350 EU/ml in solution used for aerosols) with both (two) purification protocols. Bacterial endotoxins were also detected in aerosolized form, but below the occupational exposure limit of 90 EU/m3. Despite these concerns, no symptoms were observed in exposed humans when they were using personal protective equipment. In the future, purification protocols should be developed to reduce associated bacterial endotoxin levels in enveloped bacterial virus specimens to ensure even safer research use of surrogate viruses.
摘要：
从理论上讲，替代病毒提供了研究室内环境中病毒传播的机会，在大流行期间非常需要的理解，以安全的方式保护人类和环境。然而,替代病毒作为高浓度气溶胶对人类的安全性尚未确定。在这项研究中,在所研究的室内空间中，Phi6替代品以高浓度雾化（颗粒物质2.5～1018μgm-3）。密切关注参与者的任何症状。我们测量了用于雾化的病毒溶液的细菌内毒素浓度以及含有雾化病毒的室内空气中的浓度。此外,我们测量了不同传统病毒纯化程序对样品细菌内毒素浓度的影响。尽管净化，使用两种（两种）纯化方案，Phi6的细菌内毒素浓度很高（用于气雾剂的溶液中为350EU/ml）。还检测到雾化形式的细菌内毒素，但低于90EU/m3的职业接触限值。尽管有这些担忧,当暴露的人使用个人防护设备时，没有观察到症状。在未来,应制定纯化方案，以降低包膜细菌病毒标本中相关的细菌内毒素水平，以确保更安全的研究使用替代病毒。
