Abstract：
BACKGROUND: High-performance thin-layer chromatography (HPTLC) was developed and validated for the determination of aloe-emodin in accordance with ICH guidelines. In addition, a novel RP-UHPLC method was developed, and both methods were used to analyse the herbal extract and herbal formulation.
METHODS: Separation was carried out on a silica gel 60 F254 HPTLC plate using the mobile phase Toluene: Methanol (9:1). The linearity was good across the 800-4000 ng/spot range. Validation results are within acceptable limits. The percent RSD for accuracy was 0.58-1.77, and precision was 1.10-1.97 and 1.45-1.94 for intraday and interday, respectively. The percentage of aloe-emodin found in the herbal extract and aloe vera capsule was 99.83 ± 1.19 and 99.53 ± 1.29, respectively, using this method.
RESULTS: Quantification of aloe-emodin in herbal extract and herbal formulation were done using a novel UHPLC method with chromatographic conditions of orthophosphoric acid Methanol (0.1 percent OPA): Water (65:35, v/v) and pH 3, a flow rate of 1.2 ml/min, and elute detection at 254 nm. At 6.32 minutes, a sharp and symmetric peak was observed. The method developed was validated in accordance with ICH guidelines. The percent RSD numerical value of accuracy was 0.304-0.576, and the inter-day and intraday precision were 0.32-3.08 and 0.51-2.78, respectively. Herbal extract and aloe vera capsule were analysed using the new UHPLC method. Aloe-emodin percentages were reported as 100.3 ± 0.89 and 99.53 ± 1.29, respectively.
CONCLUSIONS: The antimicrobial and anti-oxidant activities of an aloe-vera herbal formulation were studied, and the results were positive.
METHODS: Separation was carried out on a silica gel 60 F254 HPTLC plate using the mobile phase Toluene: Methanol (9:1). The linearity was good across the 800-4000 ng/spot range. Validation results are within acceptable limits. The percent RSD for accuracy was 0.58-1.77, and precision was 1.10-1.97 and 1.45-1.94 for intraday and interday, respectively. The percentage of aloe-emodin found in the herbal extract and aloe vera capsule was 99.83 ± 1.19 and 99.53 ± 1.29, respectively, using this method.
RESULTS: Quantification of aloe-emodin in herbal extract and herbal formulation were done using a novel UHPLC method with chromatographic conditions of orthophosphoric acid Methanol (0.1 percent OPA): Water (65:35, v/v) and pH 3, a flow rate of 1.2 ml/min, and elute detection at 254 nm. At 6.32 minutes, a sharp and symmetric peak was observed. The method developed was validated in accordance with ICH guidelines. The percent RSD numerical value of accuracy was 0.304-0.576, and the inter-day and intraday precision were 0.32-3.08 and 0.51-2.78, respectively. Herbal extract and aloe vera capsule were analysed using the new UHPLC method. Aloe-emodin percentages were reported as 100.3 ± 0.89 and 99.53 ± 1.29, respectively.
CONCLUSIONS: The antimicrobial and anti-oxidant activities of an aloe-vera herbal formulation were studied, and the results were positive.
摘要：
背景：根据ICH指南开发并验证了高效薄层色谱法（HPTLC）用于测定芦荟大黄素。此外,开发了一种新的RP-UHPLC方法，两种方法均用于分析草药提取物和草药配方。
方法:使用流动相甲苯:甲醇(9:1)在硅胶60F254HPTLC板上进行分离。在800-4000ng/点范围内线性良好。验证结果在可接受的范围内。精度的RSD百分比为0.58-1.77，盘中和盘中的精度为1.10-1.97和1.45-1.94，分别。在草药提取物和芦荟胶囊中发现的芦荟大黄素的百分比分别为99.83±1.19和99.53±1.29，使用这种方法。
结果：使用新的UHPLC方法对草药提取物和草药制剂中的芦荟大黄素进行定量，色谱条件为正磷酸甲醇（0.1％OPA）：水（65：35，v/v）和pH3，流速为1.2ml/min，并在254nm处洗脱检测。在6.32分钟，观察到尖锐和对称的峰。所开发的方法根据ICH指南进行了验证。准确度的百分比RSD数值为0.304-0.576，日内和日内精度分别为0.32-3.08和0.51-2.78。使用新的UHPLC方法分析了草药提取物和芦荟胶囊。芦荟大黄素百分比分别为100.3±0.89和99.53±1.29。
结论：研究了芦荟草药制剂的抗菌和抗氧化活性，结果是积极的。
方法:使用流动相甲苯:甲醇(9:1)在硅胶60F254HPTLC板上进行分离。在800-4000ng/点范围内线性良好。验证结果在可接受的范围内。精度的RSD百分比为0.58-1.77，盘中和盘中的精度为1.10-1.97和1.45-1.94，分别。在草药提取物和芦荟胶囊中发现的芦荟大黄素的百分比分别为99.83±1.19和99.53±1.29，使用这种方法。
结果：使用新的UHPLC方法对草药提取物和草药制剂中的芦荟大黄素进行定量，色谱条件为正磷酸甲醇（0.1％OPA）：水（65：35，v/v）和pH3，流速为1.2ml/min，并在254nm处洗脱检测。在6.32分钟，观察到尖锐和对称的峰。所开发的方法根据ICH指南进行了验证。准确度的百分比RSD数值为0.304-0.576，日内和日内精度分别为0.32-3.08和0.51-2.78。使用新的UHPLC方法分析了草药提取物和芦荟胶囊。芦荟大黄素百分比分别为100.3±0.89和99.53±1.29。
结论：研究了芦荟草药制剂的抗菌和抗氧化活性，结果是积极的。
