Abstract:
In atherosclerosis, chronic inflammatory processes in local diseased areas may lead to the accumulation of reactive oxygen species (ROS). In this study, we devised a highly sensitive H2O2-scavenging nano-bionic system loaded with probucol (RPP-PU), to treat atherosclerosis more effectively. The RPP material had high sensitivity to H2O2, and the response sensitivity could be reduced from 40 to 10 μmol/L which was close to the lowest concentration of H2O2 levels of the pathological environment. RPP-PU delayed the release and prolonged the duration of PU in vivo. In Apolipoprotein E deficient (ApoE‒/‒) mice, RPP-PU effectively eliminated pathological ROS, reduced the level of lipids and related metabolic enzymes, and significantly decreased the area of vascular plaques and fibers. Our study demonstrated that the H2O2-scavenging nano-bionic system could scavenge the abundant ROS in the atherosclerosis lesion, thereby reducing the oxidative stress for treating atherosclerosis and thus achieve the therapeutic goals with atherosclerosis more desirably.
摘要:
在动脉粥样硬化中,局部患病区域的慢性炎症过程可能导致活性氧(ROS)的积累。在这项研究中,我们设计了一种高灵敏的H2O2清除纳米仿生系统,负载普罗布考(RPP-PU),更有效地治疗动脉粥样硬化.RPP材料对H2O2具有较高的敏感性,反应灵敏度可从40μmol/L降至10μmol/L,接近病理环境中H2O2的最低浓度水平。RPP-PU延缓了PU的释放并延长了其在体内的持续时间。在载脂蛋白E缺陷(ApoE-/-)小鼠中,RPP-PU有效消除病理性ROS,降低脂质和相关代谢酶的水平,并显著减少血管斑块和纤维的面积。我们的研究表明,清除H2O2的纳米仿生系统可以清除动脉粥样硬化病变中丰富的ROS,从而减少用于治疗动脉粥样硬化的氧化应激,并因此更理想地实现动脉粥样硬化的治疗目标。