Abstract：
Climate change has increased the risk for infection of crops with insect-transmitted viruses. Mild autumns provide prolonged active periods to insects, which may spread viruses to winter crops. In autumn 2018, green peach aphids (Myzus persicae) were found in suction traps in southern Sweden that presented infection risk for winter oilseed rape (OSR; Brassica napus) with turnip yellows virus (TuYV). A survey was carried out in spring 2019 with random leaf samples from 46 OSR fields in southern and central Sweden using DAS-ELISA, and TuYV was detected in all fields except one. In the counties of Skåne, Kalmar, and Östergötland, the average incidence of TuYV-infected plants was 75%, and the incidence reached 100% for nine fields. Sequence analyses of the coat protein gene revealed a close relationship between TuYV isolates from Sweden and other parts of the world. High-throughput sequencing for one of the OSR samples confirmed the presence of TuYV and revealed coinfection with TuYV-associated RNA. Molecular analyses of seven sugar beet (Beta vulgaris) plants with yellowing, collected in 2019, revealed that two of them were infected by TuYV, together with two other poleroviruses: beet mild yellowing virus and beet chlorosis virus. The presence of TuYV in sugar beet suggests a spillover from other hosts. Poleroviruses are prone to recombination, and mixed infection with three poleroviruses in the same plant poses a risk for the emergence of new polerovirus genotypes. [Formula: see text] Copyright © 2023 The Author(s). This is an open access article distributed under the CC BY 4.0 International license.
摘要：
气候变化增加了作物感染昆虫传播病毒的风险。温和的秋天为昆虫提供了延长的活跃期，这可能会将病毒传播到冬季作物上。2018年秋季，在瑞典南部的吸虫中发现了桃绿色蚜虫（Myzuspersicae），这对冬季油菜（OSR；甘蓝型油菜）和萝卜黄化病毒（TuYV）具有感染风险。2019年春季，使用DAS-ELISA对瑞典南部和中部46个OSR田地的随机叶片样本进行了调查，结果在除一个田地外的所有田地中都检测到了TuYV。在斯科恩各县，卡尔马和厄斯特格特兰，TuYV感染植物的平均发病率为75％，9个田地的发病率达到100％。外壳蛋白基因的序列分析揭示了来自瑞典和世界其他地区的TuYV分离株之间的密切关系。OSR样品之一的高通量测序证实了TuYV的存在，并揭示了与TuYV相关RNA的共感染。7种甜菜（Betavulgaris）植物变黄的分子分析，在2019年收集，发现其中两个被TuYV与另外两种病毒一起感染：甜菜轻度黄化病毒和甜菜萎黄病病毒。甜菜中TuYV的存在表明其他宿主的溢出。杆状病毒容易重组,并且在同一植物中混合感染三种polerovirus会带来新polerovirus基因型出现的风险。
