Abstract：
This study investigated the mechanism of carbapenem resistance in an Enterobacter cloacae complex positive by the modified carbapenem inactivation method (mCIM) but negative by the Rosco Neo-Rapid Carb Kit, β CARBA, and conventional PCR for common carbapenemase genes (KPC, NDM, OXA-48, IMP, VIM, GES, and IMI/NMC). Using whole genome sequencing (WGS) data we confirmed the identification of Enterobacter asburiae (ST1639) and the presence of blaFRI-8 located on a 148kb IncFII(Yp) plasmid. This is the first occurrence of a clinical isolate harboring the FRI-8 carbapenemase and the second occurrence of FRI in Canada. This study highlights the need to use both WGS and phenotypic screening methods for detection of carbapenemase-producing strains if we consider the growing diversity of carbapenemases.
摘要：
这项研究调查了通过改良的碳青霉烯灭活法（mCIM）阳性但通过RoscoNeo-RapidCarbKit阴性的阴沟肠杆菌复合物中碳青霉烯耐药的机制，βCARBA,和常见碳青霉烯酶基因的常规PCR(KPC，NDM,OXA-48,IMP,VIM,GES,和IMI/NMC)。使用全基因组测序（WGS）数据，我们证实了肠杆菌的鉴定（ST1639）和位于148kbIncFII（Yp）质粒上的blaFRI-8的存在。这是首次出现携带FRI-8碳青霉烯酶的临床分离株，也是加拿大第二次出现FRI。这项研究强调，如果我们考虑碳青霉烯酶的多样性不断增长，则需要同时使用WGS和表型筛选方法来检测产生碳青霉烯酶的菌株。
