{Reference Type}: Journal Article
{Title}: Genetic and Phenotypic Characterization of the First Canadian Case of Ambler Class A Carbapenemase FRI-8.
{Author}: Mataseje LF;Doualla-Bell F;Boyd DA;Fakharuddin K;Felipe Garcia Jeldes H;Plante V;Beaudoin MC;Godbout A;Wong S;Fafard J;Mulvey MR;
{Journal}: Microb Drug Resist
{Volume}: 29
{Issue}: 2
{Year}: Feb 2023
{Factor}: 2.706
{DOI}: 10.1089/mdr.2022.0123
{Abstract}: This study investigated the mechanism of carbapenem resistance in an Enterobacter cloacae complex positive by the modified carbapenem inactivation method (mCIM) but negative by the Rosco Neo-Rapid Carb Kit, β CARBA, and conventional PCR for common carbapenemase genes (KPC, NDM, OXA-48, IMP, VIM, GES, and IMI/NMC). Using whole genome sequencing (WGS) data we confirmed the identification of Enterobacter asburiae (ST1639) and the presence of blaFRI-8 located on a 148kb IncFII(Yp) plasmid. This is the first occurrence of a clinical isolate harboring the FRI-8 carbapenemase and the second occurrence of FRI in Canada. This study highlights the need to use both WGS and phenotypic screening methods for detection of carbapenemase-producing strains if we consider the growing diversity of carbapenemases.