Abstract：
Borrelia burgdorferi sensu lato, the causative agents of Lyme borreliosis, are transmitted by Ixodes ticks. Tick saliva proteins are instrumental for survival of both the vector and spirochete and have been investigated as targets for vaccine targeting the vector. In Europe, the main vector for Lyme borreliosis is Ixodes ricinus, which predominantly transmits Borrelia afzelii. We here investigated the differential production of I. ricinus tick saliva proteins in response to feeding and B. afzelii infection.
Label-free Quantitative Proteomics and Progenesis QI software was used to identify, compare, and select tick salivary gland proteins differentially produced during tick feeding and in response to B. afzelii infection. Tick saliva proteins were selected for validation, recombinantly expressed and used in both mouse and guinea pig vaccination and tick-challenge studies.
We identified 870 I. ricinus proteins from which 68 were overrepresented upon 24-hours of feeding and B. afzelii infection. Selected tick proteins were successfully validated by confirming their expression at the RNA and native protein level in independent tick pools. When used in a recombinant vaccine formulation, these tick proteins significantly reduced the post-engorgement weights of I. ricinus nymphs in two experimental animal models. Despite the reduced ability of ticks to feed on vaccinated animals, we observed efficient transmission of B. afzelii to the murine host.
Using quantitative proteomics, we identified differential protein production in I. ricinus salivary glands in response to B. afzelii infection and different feeding conditions. These results provide novel insights into the process of I. ricinus feeding and B. afzelii transmission and revealed novel candidates for an anti-tick vaccine.
Label-free Quantitative Proteomics and Progenesis QI software was used to identify, compare, and select tick salivary gland proteins differentially produced during tick feeding and in response to B. afzelii infection. Tick saliva proteins were selected for validation, recombinantly expressed and used in both mouse and guinea pig vaccination and tick-challenge studies.
We identified 870 I. ricinus proteins from which 68 were overrepresented upon 24-hours of feeding and B. afzelii infection. Selected tick proteins were successfully validated by confirming their expression at the RNA and native protein level in independent tick pools. When used in a recombinant vaccine formulation, these tick proteins significantly reduced the post-engorgement weights of I. ricinus nymphs in two experimental animal models. Despite the reduced ability of ticks to feed on vaccinated animals, we observed efficient transmission of B. afzelii to the murine host.
Using quantitative proteomics, we identified differential protein production in I. ricinus salivary glands in response to B. afzelii infection and different feeding conditions. These results provide novel insights into the process of I. ricinus feeding and B. afzelii transmission and revealed novel candidates for an anti-tick vaccine.
摘要：
背景：伯氏疏螺旋体，莱姆病的病原体，由Ixodes滴答传输。滴答唾液蛋白有助于载体和螺旋体的存活，并已作为靶向载体的疫苗的靶标进行了研究。在欧洲,莱姆病的主要媒介是蓖麻，主要传播afzelii伯氏螺旋体。我们在这里调查了因进食和阿夫泽利芽孢杆菌感染而产生的蓖麻蜱唾液蛋白的差异。
方法：使用无标记定量蛋白质组学和前发生QI软件鉴定，比较,并选择在tick食过程中差异产生的tick唾液腺蛋白，以及对afzelii感染的反应。选择唾液蛋白进行验证，重组表达并用于小鼠和豚鼠疫苗接种和蜱攻击研究。
结果：我们鉴定出870种蓖麻蛋白，其中68种蛋白在喂养24小时和阿非泽利芽孢杆菌感染后被高估。通过确认它们在RNA和天然蛋白质水平上在独立的蜱池中的表达,成功地验证了所选择的蜱蛋白。当用于重组疫苗制剂时，在两个实验动物模型中，这些蜱蛋白显著降低了蓖麻若虫的充血后体重。尽管蜱以接种疫苗的动物为食的能力降低，我们观察到B.afzelii有效传播到鼠宿主。
结论：使用定量蛋白质组学，我们确定了蓖麻唾液腺中蛋白质产量的差异，以响应阿夫兹利芽孢杆菌感染和不同的喂养条件。这些结果提供了对I.ricinus喂食和B.afzelii传播过程的新见解，并揭示了抗蜱疫苗的新候选物。
方法：使用无标记定量蛋白质组学和前发生QI软件鉴定，比较,并选择在tick食过程中差异产生的tick唾液腺蛋白，以及对afzelii感染的反应。选择唾液蛋白进行验证，重组表达并用于小鼠和豚鼠疫苗接种和蜱攻击研究。
结果：我们鉴定出870种蓖麻蛋白，其中68种蛋白在喂养24小时和阿非泽利芽孢杆菌感染后被高估。通过确认它们在RNA和天然蛋白质水平上在独立的蜱池中的表达,成功地验证了所选择的蜱蛋白。当用于重组疫苗制剂时，在两个实验动物模型中，这些蜱蛋白显著降低了蓖麻若虫的充血后体重。尽管蜱以接种疫苗的动物为食的能力降低，我们观察到B.afzelii有效传播到鼠宿主。
结论：使用定量蛋白质组学，我们确定了蓖麻唾液腺中蛋白质产量的差异，以响应阿夫兹利芽孢杆菌感染和不同的喂养条件。这些结果提供了对I.ricinus喂食和B.afzelii传播过程的新见解，并揭示了抗蜱疫苗的新候选物。
