Proanthocyanidin oligomers extract from hawthorn mediates cell cycle arrest, apoptosis, and lysosome vacuolation on HCT116 cells.

山楂原花青素低聚物提取物介导细胞周期阻滞 ，凋亡 ，和 HCT116 细胞上的溶酶体液泡化。

• 影响指数: 6.269
• 发表时间: 2022
• 来源期刊: Curr Res Food Sci
• DOI: 10.1016/j.crfs.2022.05.009
• PMID: 36686366
• 文章类型: Journal Article
• 作者列表: Sun YS,Wang ZW,Gao Z,Zhao W,Thakur K,Zhong Q,Wei ZJ

关键词: Apoptosis; Cell cycle; Colon cancer; Hawthorn proanthocyanidin oligomers extract; Lysosomes; Vacuolation

Abstract

In this study, Hawthorn oligomic procyanidins extracts (HPOE) were evaluated for their anticancer activity on colorectal cancer. Our results showed that HPOE arrested HCT116 cells cycle at G2/M phase through P53-Cyclin B pathway and promoted apoptosis partly via mitochondrial (Caspase 9-Caspase 3) and death receptor (Caspase 8-Caspase 3) pathways. Meanwhile, it was found that HPOE aggravated HCT116 cells death through lysosomal vacuolation, which was verified by inhibitor/activator of P53-ILC3 signaling pathway. Taken together, HPOE exerted anticancer effects which lays the foundation for the development of functional foods for clinical colon cancer patients.

摘要

在这项研究中,评估了山楂寡聚原花青素提取物（HPOE）对结直肠癌的抗癌活性。我们的结果表明，HPOE通过P53-CyclinB途径将HCT116细胞周期阻滞在G2/M期，并部分通过线粒体（Caspase9-Caspase3）和死亡受体（Caspase8-Caspase3）途径促进细胞凋亡。同时,发现HPOE通过溶酶体液泡化加重HCT116细胞死亡，通过P53-ILC3信号通路的抑制剂/激活剂验证。一起来看,HPOE发挥抗癌作用，为临床结肠癌患者开发功能性食品奠定了基础。