Abstract：
Uropathogenic Escherichia coli (UPEC) are causative agent that causes urinary tract infections (UTIs) and the recent emergence of multidrug resistance (MDR) of UPEC increases the burden on the community. Recent studies of bacterial outer membrane vesicles (OMV) identified various factors including proteins, nucleic acids, and small molecules which provided inter-cellular communication within the bacterial population. However, the components of UPEC-specific OMVs and their functional role remain unclear. Here, we systematically determined the proteomes of UPEC-OMVs and identified the specific components that provide functions to the recipient bacteria. Based on the functional network of OMVs\' proteomes, a group of signaling peptides was found in all OMVs which provide communication among bacteria. Moreover, we demonstrated that treatment with UPEC-OMVs affected the motility and biofilm formation of the recipient bacteria, and further identified aromatic amino acid (AAA) biosynthesis proteins as the key factors to provide their movement.
摘要：
尿路致病性大肠杆菌（UPEC）是引起尿路感染（UTI）的病原体，最近出现的UPEC多药耐药性（MDR）增加了社区的负担。最近对细菌外膜囊泡（OMV）的研究确定了各种因素，包括蛋白质，核酸,以及在细菌种群内提供细胞间通讯的小分子。然而,UPEC特异性OMV的成分及其功能作用尚不清楚.这里,我们系统地测定了UPEC-OMV的蛋白质组,并鉴定了为受体细菌提供功能的特定成分.基于OMV蛋白质组的功能网络，在所有提供细菌间通讯的OMV中发现了一组信号肽。此外，我们证明了用UPEC-OMV处理影响受体细菌的运动性和生物膜形成，并进一步确定了芳香族氨基酸（AAA）生物合成蛋白是提供其运动的关键因素。
