Abstract：
Development of molecular biology and understanding structures and functions of various biological molecules and entities allowed to construct various sophisticated tools for different biotechnological, medical, and veterinary applications. One of them is the phage display technology, based on the possibility to create specific bacteriophages bearing fusion genes, which code for fusion proteins consisting of a phage coat protein and a peptide of any amino acid sequence. Such proteins retain their biological functions as structural elements of phage virions while exposing foreign peptide sequences on their surfaces. Genetic manipulations allow to construct phage display libraries composed of billions of variants of exposed peptides; such libraries can be used to select peptides of desired features. Although the phage display technology has been widely used in biotechnology and medicine, its applications in veterinary and especially in poultry science were significantly less frequent. Nevertheless, many interesting discoveries have been reported also in the latter field, providing evidence for a possibility of effective applications of phage display-related methods in developing novel diagnostic tools, new vaccines, and innovative potential therapies dedicated to poultry. Especially, infectious diseases caused by avian viruses, bacteria, and unicellular eukaryotic parasites were investigated in this field. These studies are summarized and discussed in this review, with presentation of various possibilities provided by different phage display systems in development of useful and effective products facilitating management of the problem of infectious diseases of poultry.
摘要：
分子生物学的发展和理解各种生物分子和实体的结构和功能，从而为不同的生物技术构建各种复杂的工具，medical,和兽医应用。其中之一是噬菌体展示技术，基于产生带有融合基因的特定噬菌体的可能性，编码由噬菌体外壳蛋白和任何氨基酸序列的肽组成的融合蛋白。此类蛋白质保留其作为噬菌体病毒体的结构元件的生物学功能,同时在其表面上暴露外源肽序列。遗传操作允许构建由数十亿个暴露肽变体组成的噬菌体展示文库;此类文库可用于选择具有所需特征的肽。尽管噬菌体展示技术已广泛应用于生物技术和医学领域,它在兽医，特别是家禽科学中的应用明显较少。然而，在后一个领域也报道了许多有趣的发现，为噬菌体展示相关方法在开发新的诊断工具中的有效应用提供证据，新疫苗,以及致力于家禽的创新潜在疗法。尤其是,由禽类病毒引起的传染病，细菌,和单细胞真核寄生虫在这一领域进行了研究。本文对这些研究进行了总结和讨论，不同的噬菌体展示系统在开发有用和有效的产品中提供了各种可能性，以促进家禽传染病问题的管理。
