Abstract:
Exogenous applications of phage lysins against Vibrio parahaemolyticus (V. parahaemolyticus) are a challenge due to the gram-negative bacteria outer membrane barrier. This study aimed to improve the antibacterial effect of V. parahaemolyticus phage lysin Lysqdvp001 (Lys), the best-characterized lysin with lytic activity against multiple species of Vibrios, by using liposome delivery. Various kinds of Lys-loaded liposome (Lys-lip) systems were designed and tested. The antibacterial activities of cationic guar gum (CGG) containing liposomes were much higher than the other liposomes, causing >5 log10CFU/mL of reductions of V. parahaemolyticus in buffer and severely damaging the bacterial cell structure. Moreover, some CGG liposome formulations retained high antibacterial effect after both 60-80 °C heat treatments and freeze-drying. Besides, the most stable liposome formulation killed 99 % of V. parahaemolyticus in the seawater with live clams, and its depuration rate against the bacterial contaminated clams also reached 99 %.
摘要:
噬菌体溶素对副溶血性弧菌的外源应用(V。副溶血)是一个挑战,由于革兰氏阴性细菌外膜屏障。本研究旨在提高副溶血性弧菌噬菌体溶素Lysqdvp001(Lys)的抗菌作用,对多种弧菌具有裂解活性的最佳特征的溶素,通过使用脂质体递送。设计并测试了各种装载Lys的脂质体(Lys-lip)系统。含阳离子瓜尔胶(CGG)脂质体的抗菌活性远高于其他脂质体,导致缓冲液中副溶血性弧菌减少>5log10CFU/mL,并严重损害细菌细胞结构。此外,一些CGG脂质体制剂在60-80°C热处理和冷冻干燥后保持高抗菌效果。此外,最稳定的脂质体制剂在海水中杀死了99%的副溶血性弧菌,其对细菌污染蛤仔的净化率也达到了99%。