Abstract：
The current study aimed to identify the SEPW1 and JAML genes in lamb as candidate genes related to lamb odor and flavor. The polymorphism study showed that the SEPW1 gene was polymorphic at the BanI restriction site with three genotypes (AA, AG, and GG), whereas the JAML gene was monomorphic at HhaI with genotype (GG). The association of SEPW1 between genotype and lamb odor and flavor (BCFAs and skatole) was analyzed using GLM (General Linear Model). MNA (4-methylnonanoic) was significantly associated (p < 0.05) with lamb odor and flavor. AA genotype has a lower level of MNA than AG and GG, while MOA (4-methyloctanoic), EOA(4-ethyloctanoic), MI (3-methylindole) and MP (3-methylphenol) was not significantly associated with lamb odor and flavor (p > 0.05). Furthermore, to analyze the mRNA expression of SEPW1 in liver tissues, the lambs were divided into three groups based on the genotypes AA, AG, and GG, however, mRNA expression was not differentially expressed between AA, AG, and GG (p > 0.05). These results will enhance the understanding of the functions of SEPW1 gene relation to odor and flavor traits and will shed light on the polymorphism of SEPW1 gene in lamb as a candidate gene for reducing MNA in lamb.
摘要：
本研究旨在鉴定羔羊的SEPW1和JAML基因作为与羔羊气味和风味相关的候选基因。多态性研究表明，SEPW1基因在BanI限制性位点具有三种基因型（AA，AG,和GG），而JAML基因在具有基因型（GG）的HhaI处是单态的。使用GLM(一般线性模型)分析基因型与羔羊气味和风味(BCFA和粪臭素)之间的SEPW1的关联。MNA（4-甲基壬酸）与羊肉气味和风味显着相关（p<0.05）。AA基因型的MNA水平低于AG和GG，而MOA（4-甲基辛酸），EOA（4-乙基辛酸），MI(3-甲基吲哚)和MP(3-甲基苯酚)与羊肉气味和风味无显著相关性(p>0.05)。此外，分析肝组织中SEPW1的mRNA表达，根据基因型AA将羔羊分为三组，AG,GG,然而,mRNA表达在AA、AG,和GG(p>0.05)。这些结果将增强对SEPW1基因与气味和风味性状关系的功能的理解，并将阐明羔羊SEPW1基因作为减少羔羊MNA的候选基因的多态性。
