Abstract：
Hybrid chains are a combination of ubiquitin (Ub) and Ub-like (UbL) proteins, expanding on the finely tuned Ub code. To decipher this intricate code, understanding of its assembly, architecture, as well as specific interactors of these Ub/UbL hybrid chains are important, warranting the development of suitable reagents. Here, we describe the chemical methodology to access linkage specific non-hydrolyzable Ub-NEDD8-based chains endowed with an affinity handle in all possible combinations of K48 hybrid chain dimers between Ub and NEDD8.
摘要：
混合链是泛素（Ub）和Ub样（UbL）蛋白的组合，在微调的Ub代码上扩展。为了破译这个复杂的代码，理解它的集合，architecture,以及这些Ub/UbL混合链的特定相互作用者也很重要，保证开发合适的试剂。这里,我们描述了在Ub和NEDD8之间的K48杂化链二聚体的所有可能组合中获得具有亲和处理的基于连接特异性不可水解Ub-NEDD8的链的化学方法。
