{Reference Type}: Journal Article
{Title}: Chemical Synthesis of Non-hydrolyzable Ubiquitin(-Like) Hybrid Chains.
{Author}: Pérez Berrocal DA;van der Heden van Noort GJ;Mulder MPC;
{Journal}: Methods Mol Biol
{Volume}: 2602
{Issue}: 0
{Year}: 2023
暂无{DOI}: 10.1007/978-1-0716-2859-1_3
{Abstract}: Hybrid chains are a combination of ubiquitin (Ub) and Ub-like (UbL) proteins, expanding on the finely tuned Ub code. To decipher this intricate code, understanding of its assembly, architecture, as well as specific interactors of these Ub/UbL hybrid chains are important, warranting the development of suitable reagents. Here, we describe the chemical methodology to access linkage specific non-hydrolyzable Ub-NEDD8-based chains endowed with an affinity handle in all possible combinations of K48 hybrid chain dimers between Ub and NEDD8.