%0 Journal Article
%T Chemical Synthesis of Non-hydrolyzable Ubiquitin(-Like) Hybrid Chains.
%A Pérez Berrocal DA
%A van der Heden van Noort GJ
%A Mulder MPC
%J Methods Mol Biol
%V 2602
%N 0
%D 2023
%M 36446965
暂无%R 10.1007/978-1-0716-2859-1_3
%X Hybrid chains are a combination of ubiquitin (Ub) and Ub-like (UbL) proteins, expanding on the finely tuned Ub code. To decipher this intricate code, understanding of its assembly, architecture, as well as specific interactors of these Ub/UbL hybrid chains are important, warranting the development of suitable reagents. Here, we describe the chemical methodology to access linkage specific non-hydrolyzable Ub-NEDD8-based chains endowed with an affinity handle in all possible combinations of K48 hybrid chain dimers between Ub and NEDD8.