Abstract：
We induced experimental nephrolithiasis in female rats using ethylene glycol (EG) and ammonium chloride (AC). We investigated the effects of carvacrol, an essential oil with antioxidant and anti-inflammatory properties, on nephrolithiasis using histopathology, immunohistochemistry and biochemistry. We used 40 female rats divided into four equal groups: control group, administered olive oil; carvacrol group, administered carvacrol in olive oil; nephrolithiasis group, administered EG and AC to induce experimental nephrolithiasis; treatment group with induced nephrolithiasis and administered carvacrol in olive oil. We observed no significant difference in crystal accumulation in the treatment group compared to the nephrolithiasis group. We found a significant reduction in hydropic degeneration of tubules and degree of inflammatory cell infiltration of intertubule areas. We also found a significant reduction in immunohistochemical staining of macrophage- and monocyte-specific antigens. Carvacrol treatment reversed the induced nephrolithiasis, increased malondialdehyde and urea, and decreased levels of glutathione peroxidase and catalase. Although carvacrol did not decrease crystal accumulation, it reduced pathological and biochemical damage, and improved kidney function by lowering the serum urea level.
摘要：
我们使用乙二醇（EG）和氯化铵（AC）在雌性大鼠中诱发实验性肾结石。我们调查了香芹酚的作用,一种具有抗氧化和抗炎特性的精油，使用组织病理学研究肾结石，免疫组织化学和生物化学。我们使用40只雌性大鼠，分为4组：对照组，给予橄榄油；香芹酚组，在橄榄油中服用香芹酚；肾结石组，给予EG和AC诱导实验性肾结石；治疗组诱导肾结石，并在橄榄油中给予香芹酚。与肾结石组相比，我们观察到治疗组的晶体积累没有显着差异。我们发现小管的水肿变性和管间区域的炎性细胞浸润程度显着减少。我们还发现巨噬细胞和单核细胞特异性抗原的免疫组织化学染色显着降低。香芹酚治疗逆转了诱发的肾结石，丙二醛和尿素增加，谷胱甘肽过氧化物酶和过氧化氢酶水平降低。虽然香芹酚没有减少晶体积累，它减少了病理和生化损伤，通过降低血清尿素水平改善肾功能。
