Abstract：
A novel peptide Ser-Asp-Asp-Val-Leu (SDDVL) of excellent zinc-chelating capacity (13.77 mg/g) was identified in millet bran protein hydrolysates. In silico prediction demonstrated that SDDVL had no potential toxicity. The results of structural characterization demonstrated that both amino group and carboxyl group of SDDVL were the primary zinc-chelating sites. Moreover, SDDVL-zinc chelate showed higher stability (p < 0.05) than ZnSO4 and zinc gluconate under different processing conditions including most pasteurization conditions, heating at 100°C for 10-50 min, various pH values (8.0-10.0), treatment of glucose (4-8 g/100 g) or NaCl (1-4 g/100 g), and simulated gastrointestinal digestion. In addition, SDDVL-zinc chelate showed higher zinc transport capacity than ZnSO4 and zinc gluconate in Caco-2 cells (p < 0.05). These results suggested that millet bran peptide had a positive effect on the gastrointestinal stability and bioavailability of Zn, and SDDVL-zinc chelate could be used as ingredient of zinc supplements. PRACTICAL APPLICATION: The current study provided a practical method to identify peptides of excellent zinc-chelating capacity from millet bran protein hydrolysates. This study demonstrated that in silico prediction assisted with suitable database was a fast, practical, and economic way to evaluate the security and to analysis the physicochemical properties of novel peptides. Moreover, it provided an efficient method to assess the stability of peptide-zinc chelate under different food processing conditions, which was the theoretical basis for utilization of peptide as ingredient of zinc fortifications.
摘要：
在小米麸皮蛋白水解物中鉴定出具有优异锌螯合能力（13.77mg/g）的新型肽Ser-Asp-Asp-Val-Leu（SDDVL）。计算机预测表明SDDVL没有潜在的毒性。结构表征结果表明，SDDVL的氨基和羧基都是主要的锌螯合位点。此外，SDDVL-锌螯合物在包括大多数巴氏灭菌条件在内的不同加工条件下显示出比ZnSO4和葡萄糖酸锌更高的稳定性（p<0.05）。在100°C下加热10-50分钟，各种pH值（8.0-10.0），处理葡萄糖（4-8g/100g）或NaCl（1-4g/100g），和模拟胃肠消化。此外,SDDVL-锌螯合物在Caco-2细胞中显示出比ZnSO4和葡萄糖酸锌更高的锌转运能力(p<0.05)。这些结果表明，小米麸皮肽对Zn的胃肠道稳定性和生物利用度具有积极作用，SDDVL-锌螯合物可用作锌补充剂的成分。实际应用:目前的研究提供了一种从小米麸皮蛋白水解物中鉴定具有优异锌螯合能力的肽的实用方法。这项研究表明，在适当的数据库辅助下进行计算机预测是一种快速的，实用,用经济的方法对新型多肽进行安全性评价和理化性质分析。此外，它提供了一种有效的方法来评估肽锌螯合物在不同食品加工条件下的稳定性，这是利用肽作为锌防御工事成分的理论基础。
