RESULTS: The breast cell lines were exposed to 50 Hz ELF-MF at flux densities of 0.1 mT and 1.0 mT and were examined 96 h after the beginning of ELF-MF exposure. The duration of 50 Hz ELF-MF exposure influenced the cell viability and proliferation of both the tumor and nontumorigenic breast cell lines. In particular, short-term exposure (4-8 h, 0.1 mT and 1.0 mT) led to an increase in viability in breast cancer cells, while long and high exposure (24 h, 1.0 mT) led to a decrease in viability and proliferation in all cell lines. Cancer and normal breast cells exhibited different responses to ELF-MF. Mitochondrial membrane potential and reactive oxygen species (ROS) production were altered after ELF-MF exposure, suggesting that the mitochondria are a probable target of ELF-MF in breast cells.
CONCLUSIONS: The viability of breast cells in vitro is influenced by ELF-MF exposure at magnetic flux densities compatible with the limits for the general population and for workplace exposures. The effects are apparent after 96 h and are related to the ELF-MF exposure time.
结果:将乳腺细胞系以0.1mT和1.0mT的通量密度暴露于50HzELF-MF,并在ELF-MF暴露开始后96小时进行检查。50HzELF-MF暴露的持续时间会影响肿瘤和非致瘤性乳腺细胞系的细胞活力和增殖。特别是,短期暴露(4-8小时,0.1mT和1.0mT)导致乳腺癌细胞的生存力增加,长时间和高暴露(24小时,1.0mT)导致所有细胞系的活力和增殖降低。癌症和正常乳腺细胞对ELF-MF表现出不同的反应。ELF-MF暴露后,线粒体膜电位和活性氧(ROS)的产生发生了变化,表明线粒体是乳腺细胞中ELF-MF的可能靶标。
结论:在与普通人群和工作场所暴露的限制相一致的磁通量密度下,ELF-MF暴露会影响乳腺细胞的体外活力。96小时后效果明显,与ELF-MF暴露时间有关。