■海洋藻类正日益成为新药的潜在资源。近几十年来,包括Nitida(B.Nitida)。同时,抗微生物和抗癌剂是开发替代化合物的首选,考虑到每年增加的抗性事件。因此,本研究的目的是检查双歧杆菌分离化合物的抗微生物和细胞毒性潜力。
■B.nitida产生2种化合物,谷甾醇3β四五烷酸酯和(E)-17-(8-乙基-4,5,9-三甲基十二-6-烯-2-基)-13-甲基-2,3,4,7,8,9,10,11,12,13,14,15,16,17-十四烷-氢-1H-环戊酸[a]菲-3-醇。测试两种化合物对铜绿假单胞菌具有抗菌作用,枯草芽孢杆菌,金黄色葡萄球菌,耐甲氧西林金黄色葡萄球菌(MRSA)。使用PrestoBlue™细胞活力试剂进行增殖测定,它也用于测量对MCF-7乳腺癌细胞的IC50。
■结果表明,(E)-17-(8-乙基-4,5,9-三甲基十二烷基-6-烯-2-基)-13-甲基-2,3,4,7,8,9,10,11,12,13,15,16,17-十四氢-1H-环戊酸[a]phenanthren-3-ol对金黄色葡萄球菌具有抗菌而谷甾醇3β四烷酸酯不具有任何抗菌活性,IC50值为681.65µg/mL。此外,还评估了与caspase-3受体对接诱导细胞凋亡的机制预测。
■根据结果,(E)-17-(8-乙基-4,5,9-三甲基十二-6-烯-2-基)-13-甲基-2,3,4,7,8,9,10,11,12,13,14,15,16,17-十四氢-1H-环戊烷[a]酚作为抗微生物剂和抗癌剂具有巨大潜力。
UNASSIGNED: Marine algae are increasingly becoming a potential resource for new drugs. In recent decades, including Bornetella nitida (B. nitida). Meanwhile, antimicrobial and anticancer agents are the first line of choice for developing alternative compounds, considering the annually increasing resistant events. Therefore, this study aimed to examine the antimicrobial and cytotoxic potential of B. nitida isolate compounds.
UNASSIGNED: The B. nitida resulted in 2 compounds, sitosterol 3β tetracosanoate and (E)-17-(8-ethyl-4,5,9-trimethyldec-6-en-2-yl)-13-methyl-2,3,4,7,8,9,10,11,12,13,14,15,16,17-tetradecahydro-1H-cyclopenta[a]phenanthren-3-ol. Both compounds were tested to have antibacterial effects against Pseudomonas aeruginosa, Bacillus subtilis, Staphylococcus aureus, Methicillin-resistant Staphylococcus Aureus (MRSA). Proliferation assay was conducted using the PrestoBlue™ Cell Viability Reagent, which was also used to measure the IC50 against MCF-7 breast cancer cells.
UNASSIGNED: The results showed that (E)-17-(8-ethyl-4,5,9-trimethyldec-6-en-2-yl)-13-methyl-2,3,4,7,8,9,10,11,12,13,14,15,16,17-tetradecahydro-1H-cyclopenta[a]phenanthren-3-ol had antimicrobial activity against Staphylococcus aureus and IC50 value of 142.18 µg/mL against MCF-7 cells, while sitosterol 3β tetracosanoate does not have any antimicrobial activity and IC50 value of 681.65 µg/mL. Moreover, the mechanism prediction using docking with caspase-3 receptor to induce apoptosis was also evaluated.
UNASSIGNED: Based on the results, (E)-17-(8-ethyl-4,5,9-trimethyldec-6-en-2-yl)-13-methyl-2,3,4,7,8,9,10,11,12,13,14,15,16,17-tetradecahydro-1H-cyclopenta[a]phenanthren-3-ol of B. nitida has great potential as an antimicrobial and anticancer agent.