关键词: Artificial thymic organoid Gene perturbation Hematopoietic stem cell Human In vitro Lineage differentiation OP9 co-culture T cell development Viral transduction

Mesh : Humans Cell Differentiation Coculture Techniques Intercellular Signaling Peptides and Proteins / metabolism T-Lymphocytes / metabolism

来  源:   DOI:10.1007/978-1-0716-2740-2_21

Abstract:
Not only is human T cell development characterized by unique changes in surface marker expression, but it also requires specific growth factors and conditions to mimic and study T cell development in vitro. In this chapter, we provide an overview of the specific aspects that need attention when performing T cell differentiation cultures with human hematopoietic and T cell progenitors.
摘要:
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