{Reference Type}: Journal Article {Title}: In Vitro Model Systems to Study Human T Cell Development. {Author}: Lambrechts N;Liang KL;Velghe I;Strubbe S;Dolens AC;Taghon T; {Journal}: Methods Mol Biol {Volume}: 2580 {Issue}: 0 {Year}: 2023 暂无{DOI}: 10.1007/978-1-0716-2740-2_21 {Abstract}: Not only is human T cell development characterized by unique changes in surface marker expression, but it also requires specific growth factors and conditions to mimic and study T cell development in vitro. In this chapter, we provide an overview of the specific aspects that need attention when performing T cell differentiation cultures with human hematopoietic and T cell progenitors.