PDF(Pubmed)
Abstract：
UNASSIGNED: The role of osteopontin (OPN) following severe injury remains to be elucidated, especially its relationship with type I collagen (encoded by the Col1a1 gene) secretion by newly-differentiated odontoblast-like cells (OBLCs). In this study, we examined the role of OPN in the process of reparative dentin formation with a focus on reinnervation and revascularization after tooth replantation in Opn knockout (KO) and wild-type (WT) mice.
UNASSIGNED: Maxillary first molars of 2- and 3-week-old-Opn KO and WT mice (Opn KO 2W, Opn KO 3W, WT 2W, and WT 3W groups) were replanted, followed by fixation 3-56 days after operation. Following micro-computed tomography analysis, the decalcified samples were processed for immunohistochemistry for Ki67, Nestin, PGP 9.5, and CD31 and in situ hybridization for Col1a1.
UNASSIGNED: An intense inflammatory reaction occurred to disrupt pulpal healing in the replanted teeth of the Opn KO 3W group, whereas dental pulp achieved healing in the Opn KO 2W and WT groups. The tertiary dentin in the Opn KO 3W group was significantly decreased in area compared with the Opn KO 2W and WT groups, with a significantly low percentage of Nestin-positive, newly-differentiated OBLCs during postoperative days 7-14. In the Opn KO 3W group, the blood vessels were significantly decreased in area and pulp healing was disturbed with a failure of pulpal revascularization and reinnervation.
UNASSIGNED: OPN is necessary for proper reinnervation and revascularization to deposit reparative dentin following severe injury within the dental pulp of erupted teeth with advanced root development.
UNASSIGNED: Maxillary first molars of 2- and 3-week-old-Opn KO and WT mice (Opn KO 2W, Opn KO 3W, WT 2W, and WT 3W groups) were replanted, followed by fixation 3-56 days after operation. Following micro-computed tomography analysis, the decalcified samples were processed for immunohistochemistry for Ki67, Nestin, PGP 9.5, and CD31 and in situ hybridization for Col1a1.
UNASSIGNED: An intense inflammatory reaction occurred to disrupt pulpal healing in the replanted teeth of the Opn KO 3W group, whereas dental pulp achieved healing in the Opn KO 2W and WT groups. The tertiary dentin in the Opn KO 3W group was significantly decreased in area compared with the Opn KO 2W and WT groups, with a significantly low percentage of Nestin-positive, newly-differentiated OBLCs during postoperative days 7-14. In the Opn KO 3W group, the blood vessels were significantly decreased in area and pulp healing was disturbed with a failure of pulpal revascularization and reinnervation.
UNASSIGNED: OPN is necessary for proper reinnervation and revascularization to deposit reparative dentin following severe injury within the dental pulp of erupted teeth with advanced root development.
摘要：
未经授权：骨桥蛋白（OPN）在严重损伤后的作用尚待阐明，尤其是它与新分化的成牙本质细胞（OBLCs）分泌的I型胶原蛋白（由Col1a1基因编码）的关系。在这项研究中,我们研究了OPN在修复性牙本质形成过程中的作用,重点是Opn基因敲除(KO)和野生型(WT)小鼠牙齿再植后的神经支配和血运重建.
未经授权：2周龄和3周龄的OpnKO和WT小鼠的上颌第一磨牙（OpnKO2W，OpnKO3W,WT2W,和WT3W组)重新种植，术后3-56天固定。在显微计算机断层扫描分析之后，脱钙的样品被处理用于Ki67，Nestin，PGP9.5和CD31以及Col1a1的原位杂交。
未经批准：发生强烈的炎症反应，破坏了OpnKO3W组的再植牙齿的牙髓愈合，而OpnKO2W和WT组的牙髓达到愈合。与OpnKO2W和WT组相比，OpnKO3W组的三级牙本质面积明显减少，巢蛋白阳性的比例明显较低，术后7-14天新分化的OBLCs。在OpnKO3W组中，血管面积明显减少，牙髓愈合受到牙髓血运重建和神经支配失败的干扰。
UNASSIGNED：OPN对于适当的神经支配和血运重建是必要的，以在牙根发育严重的萌出牙齿牙髓内严重损伤后沉积修复性牙本质。
未经授权：2周龄和3周龄的OpnKO和WT小鼠的上颌第一磨牙（OpnKO2W，OpnKO3W,WT2W,和WT3W组)重新种植，术后3-56天固定。在显微计算机断层扫描分析之后，脱钙的样品被处理用于Ki67，Nestin，PGP9.5和CD31以及Col1a1的原位杂交。
未经批准：发生强烈的炎症反应，破坏了OpnKO3W组的再植牙齿的牙髓愈合，而OpnKO2W和WT组的牙髓达到愈合。与OpnKO2W和WT组相比，OpnKO3W组的三级牙本质面积明显减少，巢蛋白阳性的比例明显较低，术后7-14天新分化的OBLCs。在OpnKO3W组中，血管面积明显减少，牙髓愈合受到牙髓血运重建和神经支配失败的干扰。
UNASSIGNED：OPN对于适当的神经支配和血运重建是必要的，以在牙根发育严重的萌出牙齿牙髓内严重损伤后沉积修复性牙本质。
