Abstract：
The zebrafish as a model organism is well known for its versatile genetics, rapid development, and straightforward live imaging. It is an excellent model to study hematopoiesis because of its highly conserved ontogeny and gene regulatory networks. Recently developed highly specific transgenic reporter lines have allowed direct imaging and tracking of hematopoietic stem and progenitor cells (HSPCs) in live zebrafish. These reporter lines can also be used for fluorescence-activated cell sorting (FACS) of HSPCs. Similar to mammalian models, HSPCs can be transplanted to reconstitute the entire hematopoietic system of zebrafish recipients. However, the zebrafish provides unique advantages to study HSPC biology, such as transplants into embryos and high-throughput chemical screening. This chapter will outline the methods needed to identify, isolate, and transplant HSPCs in zebrafish.
摘要：
斑马鱼作为一种模式生物以其多才多艺的遗传学而闻名，快速发展,和简单的实时成像。由于其高度保守的个体发育和基因调控网络，它是研究造血的极好模型。最近开发的高度特异性转基因报道系允许对活斑马鱼中的造血干细胞和祖细胞（HSPC）进行直接成像和追踪。这些报告系也可用于HSPC的荧光激活细胞分选(FACS)。类似于哺乳动物模型，可以移植HSPCs以重建斑马鱼受体的整个造血系统。然而,斑马鱼为研究HSPC生物学提供了独特的优势，例如移植到胚胎和高通量化学筛选。本章将概述需要识别的方法，隔离,并在斑马鱼中移植HSPCs。
